Molecular characterization of three Acridoid Grasshoppers Using Optimized Protocol for the DNA extraction

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Adekoya, K.O.
Ogunkanmi, L.A.
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Book of Proceedings of the 4th UNILAG Research Conference and Fair. 8th January, 2009. University of Lagos, Akoka, Lagos. Pp232 -235
The biological information derivable from molecular approach to the study of organisms provides a better basis of judgment than those of cytogenetic or morphological studies in systematics and related fields of genetics. The procedures for the extraction of high quality DNA from different organisms and different tissues within the same organisms have been proved to vary. Three grasshoppers of the same family, Acrididae, Acrida turrita, Paracinenia luculen!a and Spathosternum pygmaeum were used for the molecular studies. A total of 26 whole insects made up of nine Acrida turrita, ten Paracinema luculenta and seven Spathosternum pygmaeum were subjected to the optimized protocol of DNA extraction using the CTAB method with slight modifications in the concentration of specific substances in the extraction buffer. The modified protocol employed yielded a high quality DNA and was found to be suitable for peR analysis. A UPGMA cluster analysis generated from this analysis classified the insects into three distinct genera. The three main clusters on the dendogram using three operon primers show 82.8% coefficient of similarity for Acrida turrita, 70.8% for Paracinema luculenta and 72.7% for Spathosternum pygll1aeulIl. The overall results reveal 53.9% similarity across the genera indicating close relationship at the family level.
Genetics , DNA extraction , Grasshopper , Cytogenetics
Adekoya, K.O. and Ogunkanmi, L.A. (2009) Molecular characterization of Three Acridoid Grasshoppers Using Optimized Protocol for the DNA extraction. Book of Proceedings of the 4th UNILAG Research Conference and Fair held at University of Lagos, Akoka.