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- ItemOpen AccessScreening of cowpea lines for drought tolerance(Genetics Society of Nigeria (GSN), 2013-10) Bolarinwa, K. A.; Ogunkanmi, L. A.; Adetumbi, J. A.; Akinyosoye, S. T.; Akande, S. R.; Amusa, O. D.Thirty-nine cowpea lines were screened for drought tolerance to provide information on cowpea lines that can be used for genetic improvement of the acceptable varieties. The experiment was carried out using wooden boxes filled with top soil. Cowpea lines were planted in three replications and the seedlings were watered daily using a small watering can of four litres for two weeks until partial emergence of the first trifoliate leaves of all the varieties was observed. Thereafter, watering was stopped for thirty five days and wilted plants in each variety were counted daily. Watering was resumed for fourteen days to ascertain regeneration potentials of each variety. Significant differences were observed among the cowpea lines for drought tolerance Stress effect was first noticed on the unifoliate leaves twelve days after watering was stopped, followed by the emerging trifoliates and finally the growing tip dried. Wilting percentage at different days after termination of watering indicated TVx 3236, NG/SA/07/132 and IT95K-193-12 to be the most susceptible to drought. The recovery percentage after watering ranged from 0% for NG/SA/01/09/004 to 100 % for IT81D-994 and Oloyin. All the cowpea lines were grouped into three. Group 1 comprises three lines (TVx3236, NG/SA/07/130 and NG/SA/01/09/004) which are highly drought susceptible while Group 2 and 3 comprise of 14 and 22 lines respectively that were tolerant at varying degrees. The screening has provided information on cowpea lines that can be used in breeding for drought tolerant cowpea varieties.
- ItemOpen AccessMolecular characterization of three Acridoid Grasshoppers Using Optimized Protocol for the DNA extraction(Book of Proceedings of the 4th UNILAG Research Conference and Fair. 8th January, 2009. University of Lagos, Akoka, Lagos. Pp232 -235, 2009-01-08) Adekoya, K.O.; Ogunkanmi, L.A.The biological information derivable from molecular approach to the study of organisms provides a better basis of judgment than those of cytogenetic or morphological studies in systematics and related fields of genetics. The procedures for the extraction of high quality DNA from different organisms and different tissues within the same organisms have been proved to vary. Three grasshoppers of the same family, Acrididae, Acrida turrita, Paracinenia luculen!a and Spathosternum pygmaeum were used for the molecular studies. A total of 26 whole insects made up of nine Acrida turrita, ten Paracinema luculenta and seven Spathosternum pygmaeum were subjected to the optimized protocol of DNA extraction using the CTAB method with slight modifications in the concentration of specific substances in the extraction buffer. The modified protocol employed yielded a high quality DNA and was found to be suitable for peR analysis. A UPGMA cluster analysis generated from this analysis classified the insects into three distinct genera. The three main clusters on the dendogram using three operon primers show 82.8% coefficient of similarity for Acrida turrita, 70.8% for Paracinema luculenta and 72.7% for Spathosternum pygll1aeulIl. The overall results reveal 53.9% similarity across the genera indicating close relationship at the family level.