Mini-STRs Screening of 12 Relatives of Hausa Origin in Northern Nigeria
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Date
2017-06
Authors
Akpan, U.U.
Adekoya, K.O.
Awe, E. T.
Garba, N
Oguncoker, G.D.
Ojo, S.G.
Journal Title
Journal ISSN
Volume Title
Publisher
Nigerian Journal of Basic and Applied Sciences
Abstract
The forensic identification of closely related individuals genetically has often been difficult probably due
to strong phenotypic concordances. Short tandem repeats (STRs) have been used in kinship tracing,
identifying missing person and skeletal remains, paternity testing and mass disasters victim
identification. This research was performed to determine the efficiency of the miniSTRsD14S1434 and
D9S1122 in differentiating between closely related persons in Nigeria. The sample consisted of twelve
individuals who self-identify as relatives. DNA extracted from blood was amplified using primers for the
two miniSTR loci and resolved subsequently on 4% Agarose gel. An initial denaturation for 3 min at
95°C with annealing temperature at 57.4°C and 30 cycles of PCR produced best results. Different
alleles of the markers D9S1122 (9, 10, 12, 13, 14,15,16) and D14S1434 (13,15,17,19,20) were
identified in the samples studied with a high degree of heterozygosity as seen in the computed
estimates of 0.8000 and 0.6364 for the D9S1122 and D14S1434 loci respectively. The gel resolutions
show a combined probability of exclusion of 0.94 for the population and 0.97 for siblings. These loci can
therefore efficiently differentiate individuals and establish family relationship in the Hausa population
and will be important for forensic identification of individuals in the population.
Description
Keywords
STRs , identification , Hausa , Nigeria , Allels
Citation
Akpan, U.U., Adekoya, K.O., Awe, E.T., Garba, N., Oguncoker, G.D. and Ojo, S.G. (2017). Mini-STRs Screening of 12 Relatives of Hausa Origin in Northern Nigeria. Nigerian Journal of Basic and Applied Science. 25(1): 48 - 57