Sulphadoxine-pyrimethamine alters the antioxidant defense system in blood of rabbit

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dc.contributor.authorAdisa, R.A.
dc.contributor.authorOgunbayo, O.A.
dc.contributor.authorOlorunsogo, O.O.
dc.contributor.authorAdemowo, O.G.
dc.date.accessioned2020-02-19T08:57:29Z
dc.date.available2020-02-19T08:57:29Z
dc.date.issued2011-12
dc.descriptionStaff publicationsen_US
dc.description.abstractSummary: Sulphadoxine-pyrimethamine (SP) despite reported resistance remains an important drug of choice for the treatment and control of malaria in most endemic areas. Exacerbation of intra-erythrocytic oxidative stress might contribute to the process of elimination of malaria parasites in the body. The effect of treatment with SP on the antioxidant defense system was investigated using rabbit as a model. Ten male rabbits were divided into two groups of five animals each. The first group was administered with normal saline and served as control. The second group received a single dose of SP (26.25mg/kg body weight). Blood samples were collected before and at 6, 12 and 24 h after drug administration. Activity of cellular enzymatic antioxidants, superoxide dismutase (SOD) and catalase (CAT), and level of reduced glutathione (GSH) were assayed using standard spectrophotometric methods. Serum lipid peroxidation was assessed by the formation of thiobarbituric acid reactive species (TBARS) while protein content was assayed by the method of Lowry et al., 1951. SOD activity was observed to increase progressively by 4.9, 63.4 and 120.8% at 6, 12 and 24 h respectively, after drug administration. Similarly, CAT activity increased by 44.5, 82.6 and 116.3% at 6, 12 and 24 h, respectively. TBARS level also increased significantly by 45.5, 118.2 and 186.4%, respectively. However, the level of GSH decreased by 41.9% at 6 h and remained so up till the 12 h, but by 24 h after drug administration, the level of the thiol substance has increased considerably up to 48.4% above the baseline level. SP treatment altered the antioxidant defense system in blood and may therefore induce oxidative stress by generating reactive oxygen species. This might play significant role in the therapeutic and adverse effects associated with the drug.en_US
dc.identifier.citationAdisa, R.A., Ogunbayo, O.A., Olorunsogo, O.O. & Ademowo, O.G. (2011). Sulphadoxine-pyrimethamine alters the antioxidant defense system in blood of rabbit. Nigerian journal of physiological sciences. Niger J Physiol Sci. 26(2):207-11.en_US
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7708
dc.language.isoenen_US
dc.publisherNigerian Journal of Physiological Sciencesen_US
dc.relation.ispartofseriesNigerian journal of physiological sciences;Vol.26(2)
dc.subjectResearch Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistryen_US
dc.subjectSulphadoxineipyrimethamineen_US
dc.subjectLipid peroxidationen_US
dc.subjectoxidative stressen_US
dc.subjectBlooden_US
dc.subjectRabbiten_US
dc.titleSulphadoxine-pyrimethamine alters the antioxidant defense system in blood of rabbiten_US
dc.typeArticleen_US
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