Antibiotic use in food animals: determination of enrofloxacin residue in chicken tissue
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West African Postgraduate College of Pharmacists
Background: Poultry farmers in Nigeria employ the use of various antibiotics with or without the guidance of veterinarians, to promote growth and prevent infections in poultry. Although antibiotics benefit most of its uses, this has led to the accumulation of toxic antibiotic residues in edible poultry products destined for human consumption. Objective: The present study was aimed at determining the residual amounts of enrofloxacin in chicken muscle samples. Method: Forty birds were obtained from commercial poultries in Lagos State and randomly allotted into 7 groups. They were humanely sacrificed, dissected and 10.0 g of muscle tissue was sectioned from each drumstick and homogenized in a porcelain mortar. 5.0 g of the homogenate was weighed and transferred into a 5 ml plain sample bottle and processed for analysis by high performance liquid chromatography to determine the amount of enrofloxacin in the tissues. Results: Enrofloxacin was detected in all samples from the four farms. The mean concentration of enrofloxacin ranged from 23.5 to 88.1 μg/g in all the 7 samples analyzed; these values were higher than the maximum residue level of 0.1 ìg/ug in Europe and 0.3 ìg/ug in the United States of America. Conclusion: This study confirmed misuse of enrofloxacin in poultry farms and emphasizes the need for stricter regulation regarding antibiotic use in poultry as well as the screening of chicken for residues before sale.
Enrofloxacin , HPLC , Maxumum Residue Limit , Poultry , Residue
Kennedy I. Amagon, 1Sunday O. Olayemi, Moshood Akinleye, Olufunsho Awodele and Bola O. Silva (2017) Antibiotic use in food animals: determination of enrofloxacin residue in chicken tissue West African Journal of Pharmacy (2017) 28 (1) 98-106