Optimization of protocols for DNA extraction and RAPD analysis in West African fonio (Digitaria exilis and Digitaria iburua ) germplasm characterization
| dc.contributor.author | Kuta, D.D. | |
| dc.contributor.author | Kwon-Ndung, E. | |
| dc.contributor.author | Dachi, S. | |
| dc.contributor.author | Bakare, O | |
| dc.contributor.author | Ogunkanmi, L.A. | |
| dc.date.accessioned | 2019-09-05T11:07:12Z | |
| dc.date.available | 2019-09-05T11:07:12Z | |
| dc.date.issued | 2005-12 | |
| dc.description.abstract | Fonio is an important indigenous grain crop of West Africa, but the extent of genetic diversity in fonio, its origin and phylogeny are not well understood. DNA markers allow precise characterization of plant germplasm accessions, but there is no literature report of their use in fonio. This paper reports the result of protocol optimization research for DNA isolation and RAPD analyses in fonio. High quality DNA was successfully isolated and RAPD was effectively used to study genetic similarity among fonio accessions. This result might stimulate the application of DNA markers to investigate the origin and phylogeny of fonio in Africa. | en_US |
| dc.identifier.citation | Kuta, D.D. Kwon-Ndung, E. Dachi, S. Bakare, O Ogunkanmi, L.A. 2005. Optimization of protocols for DNA extraction and RAPD analysis in West African fonio (Digitaria exilis and Digitaria iburua ) germplasm characterization. African Journal of Biotechnology.4(12): 1368-1371 | en_US |
| dc.identifier.issn | 1684–5315 | |
| dc.identifier.uri | https://ir.unilag.edu.ng/handle/123456789/5217 | |
| dc.language.iso | en | en_US |
| dc.publisher | Academic Journals | en_US |
| dc.subject | Digitaria spp., DNA markers, RAPD, germplasm characterization, fonio. | en_US |
| dc.title | Optimization of protocols for DNA extraction and RAPD analysis in West African fonio (Digitaria exilis and Digitaria iburua ) germplasm characterization | en_US |
| dc.type | Article | en_US |