Gas Chromatography-Mass Spectrometry and Cytotoxicity of Securidaca longepedunculata (polygalaceae) Root Bark Extract

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dc.contributor.authorLawal, R.A
dc.contributor.authorOzaslan, M.D
dc.contributor.authorOdesanmi, O.S
dc.contributor.authorEbuehi, O.A.T.
dc.contributor.authorKaragoz, I.D
dc.contributor.authorKilic, I.H
dc.date.accessioned2019-09-10T08:44:39Z
dc.date.available2019-09-10T08:44:39Z
dc.date.issued2016-03
dc.description.abstractSecuridaca longepedunculata is a savannah shrub found growing in tropical Africa. It is reputed to have more than a hundred medicinal uses and is a major component of anticancer decoctions in Nigeria. Despite its reported use as an anti-cancer plant, there is a dearth of information on the anti-cancer potential and mechanism of its anticancer activity. The present study is to identify the chemical constituents present in Securidaca longepedunculata by quantitative Gas Chromatography-Mass Spectrometry (GC-MS) analysis and determine the cytotoxic effect of the plant on Ehrlich ascites carcinoma cells. Secondary metabolites in the extract were predicted using GC-MS. In vitro cytotoxic activity was determined using the trypan blue dye exclusion assay by incubating Ehrlich ascites carcinoma cells with various concentrations of S. longepedunculata aqueous extract. Analysis by GC-MS revealed the presence of the following compounds: bis (2- ethylhexyl) phthalate (90.99%), 1-decanol (4.17%) and cyclododecane (1.86%), phenol, 2, 21 - methylene bis (1.32%), cyclopenten-4-one, 3-hydroxy-1, 2, 3, 5, 51 -pentakis (trimethylsilyloxy)- (0.89%) and phenol, 2, 4-bis (1, 1-dimethylethyl)(0.78%). Securidaca longepedunculata aqueous extract (SL) was cytotoxic to Ehrlich ascites carcinoma cells in vitro. The IC50 of SL on EAC cells was 67 µg/ml. The aqueous root-bark extract of S. longepedunculata contains bioactive agents and was cytotoxic to Ehrlich ascites carcinoma cells in vitro.en_US
dc.identifier.issn2354-337X
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/5436
dc.language.isoenen_US
dc.publisherFountain Journal of Natural and Applied Sciencesen_US
dc.subjectphthalateen_US
dc.subjectEhrlichen_US
dc.subjectMedicinalen_US
dc.subjectPlantsen_US
dc.titleGas Chromatography-Mass Spectrometry and Cytotoxicity of Securidaca longepedunculata (polygalaceae) Root Bark Extracten_US
dc.typeArticleen_US
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