Genetic variation among clostridium perfringens isolated from food and faecal specimens in Lagos

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dc.contributor.authorChukwu, E.E.
dc.contributor.authorNwakorie, F.O.
dc.contributor.authorCoker, A.O.
dc.contributor.authorAvila-campos, M.J.
dc.contributor.authorOgunsola, F.T.
dc.date.accessioned2019-12-06T08:07:25Z
dc.date.available2019-12-06T08:07:25Z
dc.date.issued2017-07-30
dc.descriptionStaff publicationen_US
dc.description.abstractBackground: Clostridium perfringens is an anaerobic Gram-positive bacterium which is commonly present in the gastrointestinal tract of man and animals and causes enteritic diseases in animals and food poisoning in humans. Previous studies have looked at the epidemiological relationship between C. perfringens isolates from outbreak source. In this study, the genetic diversity of C. perfringens strains from non-outbreak food and faecal specimens was investigated for epidemiological purposes. Methods: We analyzed thirty-eight (38) Clostridium perfringens strains isolated from food and faecal specimens in Lagos State. Bacterial identification was done using colonial morphology, Gram stain reaction, conventional biochemical tests and PCR. Genetic analysis was performed using arbitrary primed polymerase chain reaction (AP-PCR) technique with oligonucleotide primer of random sequences (OPA-3) to determine the genetic diversity of C. perfringens. The distance between the different bands produced were analyzed using numerical taxonomy and multivariate system software (NTSYS). Results: Seventeen (44.7%) C. perfringens strains showed at least one polymorphic DNA patterns when genotyped. However, this method identified polymorphisms among the C. perfringens species from which four genetic groups (1, 2, 3 and 4) were established. Conclusions: Our findings suggest that there may be faecal contamination of food products and similar clones of Clostridium perfringens may be incriminated.en_US
dc.identifier.citationChukwu E.E, Nwaokorie F.O, Coker A.O, Avila-Campos M.J. and Ogunsola F.T. (2017). Genetic variation among Clostridium perfringens isolated from food and faecal specimens in Lagos. Microbial Pathogenesis,111:232-237. doi: 10.1016/j.micpath.2017.08.031.en_US
dc.identifier.otherdoi: 10.1016/j.micpath.2017.08.031
dc.identifier.urihttps://www.google.com/search?q=Genetic+variation+among+clostridium+perfringens+isolated+from+food+and+faecal+specimens+in+lagos&oq=Genetic+variation+among+clostridium+perfringens+isolated+from+food+and+faecal+specimens+in+lagos&aqs=chrome..69i57.958j0j9&sourceid=chrome&ie=UTF-8
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7042
dc.language.isoenen_US
dc.relation.ispartofseriesMicrobial Pathogenesis;111
dc.subjectClostridium perfringensen_US
dc.subjectFooden_US
dc.subjectFaecesen_US
dc.subjectGenetic variationen_US
dc.subjectFood poisoningen_US
dc.subjectAp-37 pcren_US
dc.subjectResearch Subject Categories::MEDICINEen_US
dc.titleGenetic variation among clostridium perfringens isolated from food and faecal specimens in Lagosen_US
dc.typeArticleen_US
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