Detection of toxigenic Clostridium perfringens and Clostridium botulinum from food sold in lagos, nigeria

Simple item page
dc.contributor.authorChukwu, E.E.
dc.contributor.authorNwakorie, F.O.
dc.contributor.authorCoker, A.O.
dc.contributor.authorAvila-campos, M.J.
dc.contributor.authorSolis, R.L.
dc.contributor.authorLlanco, L.A.
dc.contributor.authorOgunsola, F.T.
dc.date.accessioned2019-12-05T09:57:33Z
dc.date.available2019-12-05T09:57:33Z
dc.date.issued2016-10-23
dc.descriptionStaff Publicationsen_US
dc.description.abstractFood-borne diseases contribute to the huge burden of sickness and death globally and in the last decade, have become more frequently reported in Africa. In line with this, food safety is becoming a significant and growing public health problem in Nigeria. Diarrhoea is a common problem in Nigeria and has been reported but there has been little data on the possibility of clostridia as aetiological agents. Clostridium species are ubiquitous in the environment and in the gastrointestinal tract of man and animals and can serve as a marker for faecal contamination. We set out to determine the potential of these foods to transmit clostridium species. A total of 220 food commodities from six local governments in Lagos State were sampled. Isolates obtained were identified based on cultural, morphological and biochemical characteristics. Toxinotyping was done using multiplex-PCR with primers specific for alpha, beta, epsilon and iota-toxin genes, enterotoxigenic cpe gene and neurotoxigenic BoNt gene. Fifty (22.7%) clostridial species were isolated of which 29 (58%) were identified as C. perfringens. Toxinotyping of the 29 strains showed that 28 (96.6%) were toxin producing C. perfringens type A while one (3.4%) was C. perfringens type D. Two (4%) C. botulinum species were isolated and identified by 16S rRNA sequencing, both harbouring BoNt/A gene. The contamination rates of food with Clostridium species show that food hygiene is a problem and Clostridium species may be a source of food borne disease in Lagos State, Nigeria.en_US
dc.identifier.citationChukwu, E.E., Nwaokorie, F.O., Coker, A.O., Avila-Campos, M.J. Solis, R.L., Llanco, L.A., Ogunsola, F.T. Detection of toxigenic Clostridium perfringens and Clostridium botulinum from food sold in Lagos, Nigeria, Anaerobe 2016; 42: 176-181en_US
dc.identifier.urihttps://www.google.com/search?q=Detection+of+toxigenic+Clostridium+perfringens+and+Clostridium+botulinum+from+food+sold+in+Lagos%2C+Nigeri&oq=Detection+of+toxigenic+Clostridium+perfringens+and+Clostridium+botulinum+from+food+sold+in+Lagos%2C+Nigeri&aqs=chrome..69i57.447j0j9&sourceid=chrome&ie=UTF-8
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7040
dc.language.isoenen_US
dc.subjectClostridium perfringensen_US
dc.subjectC. botulinumen_US
dc.subjectToxinnotypingen_US
dc.subjectMultiplex-pcren_US
dc.subjectEnterotoxigenic cpe geneen_US
dc.subjectNeurotoxigenic bont geneen_US
dc.subjectResearch Subject Categories::MEDICINEen_US
dc.titleDetection of toxigenic Clostridium perfringens and Clostridium botulinum from food sold in lagos, nigeriaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
95 - Detection of toxigenic Clostridium perfringens and Clostridium botulinum from food sold in Lagos, Nigeria.pdf
Size:
1.22 MB
Format:
Adobe Portable Document Format
Description:
Main article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Medical Microbiology and parasitology- Scholarly Publications