Carotenoid and molecular marker-based diversity assessment in tropical yellow endosperm maize inbred lines
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Date
2011-09-20
Authors
Adeyemo, O. A.
Menkir, A.
Gedil, M.
Omidiji, O.
Journal Title
Journal ISSN
Volume Title
Publisher
WFL Publisher
Abstract
Maize is a staple food crop for many people in Africa where millions of children, pregnant women and nursing mothers suffer from vitamin A
deficiency. The β-carotene found in yellow maize can be used as a primary source of provitamin A. Several tropical yellow endosperm maize inbred
lines with varying carotenoid concentrations have been identified as potential parents for breeding. Diversity assessment of these inbred lines was
conducted using 17 AFLP primer combinations, 87 SSR markers and carotenoid composition to identify parents for making crosses to develop new
inbred lines with high provitamin A carotenoids. Genetic distance (GD) values among all pairs of inbred lines varied from 0.08 to 0.64 with an average
of 0.48 for AFLPs, and from 0.007 to 0.59 with an average of 0.45 for SSRs. The Euclidean distances calculated from carotenoid composition of the
lines varied from 0.30 to 9.00 with an average of 3.27. Cluster and principal coordinate analyses based on AFLP and SSR-based GD estimates
separated the lines into grouping consistent with their pedigrees. Although the correlations of molecular marker-based clustering with carotenoidbased
clustering were not strong, molecular analysis could be used as the basis to identify diverse lines with complementary carotenoid compositions
as parents for making bi-parental crosses to increase the level of provitamin A in tropical maize. The GD estimates can also be used as the basis to
select yellow endosperm maize inbred lines for broadening and diversifying the genetic base of sources of provitamin A carotenoids in tropical yellow
endosperm maize germplasm.
Description
Keywords
Tropical maize, yellow endosperm lines, SSR markers, AFLP markers, diversity, provitamin A.
Citation
Adeyemo, O., Menkir, A., Gedil, M. and Omidiji. O. 2011. Carotenoid and molecular marker-based assessment of genetic diversity in tropical -yellow endosperm maize inbred lines. Journal of Food, Environment and Agriculture. 9 (3&4): 383-392.