Sex differences in cardiac and renal responses to a high salt diet in Sprague-Dawley rats
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Date
2019
Authors
Oloyo, Ahmed Kolade
Imaga, Ngozi O. A.
Fatope, Yemisi
Sofola, Olusoga A.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
High dietary salt intake is an important risk factor for cardiovascular and renal diseases. However, sexual
disparity exists in the response of target organs to high salt diet (HSD). To determine how sex affects cardiac and
renal functions’ response to HSD, 20 weanling Sprague-Dawley rats (10 males and 10 females) were divided into
4 groups of 5 rats each. The rats were fed a normal diet (0.3% NaCl) or HSD (8% NaCl) for 12 weeks. Fluid
balance (FB) was determined from 24 hrs water intake and voided urine. Blood pressure (BP) was measured via
arterial cannulation under anesthesia (25% w/v urethane and 1% w/v α-chloralose; 5 ml/kg, i.p). Serum levels of
troponin I, aminotransaminases, creatinine, urea, uric acid and electrolytes as well as urinary concentration of
albumin, creatinine, and electrolytes were measured using appropriate assay kits. Values are presented as mean
S.E.M, compared by two-way ANOVA and Bonferroni post Hoc test. In the male rat, HSD significantly increased
BP, serum: Troponin I, LDH and sodium (p < 0.05), urinary: albumin, sodium, potassium and FB (p < 0.05). In the
female rat, HSD increased BP, serum: troponin I, LDH, sodium and creatinine clearance (p < 0.05), urinary: albumin,
sodium and potassium (p < 0.01). However, HSD increased more, the BP, serum: Troponin I, LDH, urinary
albumin and FB in male rats, while HSD increased urinary sodium more in female rats. Basal values in male vs.
female of serum LDH and urinary albumin were significantly different. Thus, sex plays an important role in the
response of the heart and kidney to salt stress.
Description
Keywords
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry , Physiology , Pathology
Citation
Oloyo, Ahmed Kolade, Imaga, Ngozi O. A., Fatope, Yemisi, Sofola, Olusoga A..Heliyon 5 (2019) e01665