Antiproliferative and apoptosis inducing activity of markhamia tomentosa leaf extract on hela cells

Simple item page
dc.contributor.authorIbrahim, M.B.
dc.contributor.authorSowemimo, A.A.
dc.contributor.authorSpies, L.
dc.contributor.authorVan de Venter, M.
dc.contributor.authorOdukoya, O.A.
dc.contributor.authorKoekomoer, T.
dc.date.accessioned2020-01-15T12:07:20Z
dc.date.available2020-01-15T12:07:20Z
dc.date.issued2013
dc.descriptionStaff publicationsen_US
dc.description.abstractEthnopharmacological relevance: Markhamia tomentosa (Benth) K. Schum ex. Engl. (Bignoniaceae), a tree widely dispersed in West Tropical Africa, is used traditionally to treat various diseases as it possesses antimicrobial, antioxidant, analgesic, anticancer and anti-inflammatory activities. Materials and methods: This study evaluates the cytotoxic effect and underlying mechanisms of the ethanolic extract of Markhamia tomentosa on HeLa and MCF-7 cancer cell lines and non-cancerous Vero cell line. Brine shrimp lethality test was used for preliminary screening. Cytotoxicity was determined using the MTT assay and IC50 was calculated. Effect of Markhamia tomentosa on the cell cycle was monitored by flow cytometry and the apoptosis-induction capability confirmed by exposure of phosphatidylserine to the outer leaflet of the plasma membrane. Loss of mitochondrial membrane potential was analysed by flow cytometry using JC-1. Results: Markhamia tomentosa was toxic to brine shrimps with LD50 of 31.62 mg/ml. Cell viability and growth of HeLa cells was inhibited by the extract with an IC50 of 189.171.76 mg/ml at 24 h post treatment. However, no cytotoxic effect was observed in MCF-7 and Vero cell lines. The extract induced cell cycle arrest in HeLa cells in the G0/G1 phase resulting in cell death after 24 h exposure. Induction of apoptosis in HeLa cells was substantiated by Annexin V-FITC/PI double staining showing phosphatidylserine translocation and depolarisation of the mitochondrial membrane potential by flow cytometry of JC-1 stained cells. Conclusion: The ethanolic extract of Markhamia tomentosa induces G0/G1 in HeLa cells followed by induction of the intrinsic pathway of apoptosis.en_US
dc.identifier.citationIbrahim MB, Sowemimo AA, Spies L, Koekomoer T, Van de venter M, Odukoya OA. Antiproliferative and apoptosis inducing activity of markhamia tomentosa leaf extract on hela cells. Journal of Ethnopharmacology, 2013; 149, 745 - 749.en_US
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7409
dc.language.isoenen_US
dc.relation.ispartofseriesJournal of Ethnopharmacology;Vol.149
dc.subjectMarkhamia tomentosaen_US
dc.subjectApoptosisen_US
dc.subjectCytotoxicityen_US
dc.subjectHelaen_US
dc.subjectResearch Subject Categories::PHARMACY::Pharmacognosyen_US
dc.titleAntiproliferative and apoptosis inducing activity of markhamia tomentosa leaf extract on hela cellsen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Ibrahim et al 2013.pdf
Size:
712.88 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pharmacognosy- Scholarly Publications