Molecular Characterization of Auricularia Spp from South-Western Nigeria using Random Amplified Polymorphic DNA (RAPD) Markers
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Date
2018
Authors
Ekun, V.S.
Adenipekun, C.O.
Ogunkanmi, L.A.
Ojuederie, O.B.
Igwe, D.O.
Journal Title
Journal ISSN
Volume Title
Publisher
Nigerian Journal of Biotechnology
Abstract
This study was conducted to investigate the genetic diversity in 48 samples of Auricularia
species randomly collected from secondary forests in Osun (11), Oyo (10), Ondo (9), Ekiti
(8), Ogun (8) and Lagos States (8) of Nigeria. Fourteen Random Amplified Polymorphic
DNA (RAPD) markers were used for molecular characterization of Auricularia species.
Phylogenetic relations were determined by cluster analysis and Polymorphic
Information Content (PIC) and gene diversity determined using standard procedures.
The Auricularia species were grouped into 6 distinct clusters based on morphological
traits. The PIC value ranged from 0.5594 (OPH-15) to 0.7819 (OPB-12) and gene
diversity from 0.5930 (OPH-15) to 0.7977 (OPB-12). Primer OPB-12 was the most
informative for genetic diversity of Auricularia species. However, primer OPB-21 gave the
highest number of alleles while OPB-12 showed the highest range of gene diversity and
accounted for the diversity of the Auricularia species. The dendrogram and the principal
coordinate analysis exhibited similar clustered patterns, revealing that all the tested
strains could be divided into six distinct groups, each of which correlated with different
geographical regions. Molecular characterisation is essential in genetic diversity studies
and has proven useful in the classification of Auricularia spp.
Description
Keywords
Auricularia spp, Cluster analysis, Genetic diversity, Mushroom cultivation, Principal coordinate analysis, Polymorphic information content, RAPD markers
Citation
Ekun, V.S. Adenipekun, C.O. Ogunkanmi, L.A. Ojuederie, O.B. Igwe, D.O. 2018. Molecular Characterization of Auricularia Spp from South-Western Nigeria using Random Amplified Polymorphic DNA (RAPD) Markers.Nigerian Journal of Biotechnology. 35(2018): 34-43