Modulation of Erythrocyte Ghost Membrane Ca2+-Pumping ATPase of Healthy and Non-Insulin Dependent Diabetic Humans by alpha, alpha Trehalose-6- Phosphate and Extracts of Carica papaya Linn.

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dc.contributor.authorAdisa, R.A.
dc.contributor.authorOke, J.M.
dc.contributor.authorOlorunsogo, O.O.
dc.date.accessioned2020-02-12T10:27:49Z
dc.date.available2020-02-12T10:27:49Z
dc.date.issued2017
dc.descriptionStaff publicationsen_US
dc.description.abstractBackground: Although glycosylated Ca - ATPase has not been demonstrated in diabetes mellitus, but it is evident that the enzyme can be significantly glycated under experimental conditions in the presence of appreciable high levels of glucose. Objective: In this study, the hypoglycaemic agents Trehalose-6-phosphate derivative (Tp) and mature but unripe fruits of Carica papaya L (Cp) were used to modulate the activity of the erythrocyte ghost membrane (EGM) Ca2+ pumping ATPase of healthy and non-insulin dependent diabetes mellitus (NIDDM) individuals. Results: The Ca -2+ pumping ATPase in membrane of healthy and NIDDM individuals was activated maximally by 2.5mg/ml (2.6 folds) and 4mg/ml (1.7 folds) of the crude extracts of Cp respectively, while 3mg/ml of Tp optimally stimulated the membrane-bound enzyme in both healthy (2.1 folds) and NIDDM (1.5 folds) individuals, respectively. The NIDDM enzyme is less active with a lower affinity for the substrate (30.6±2.52 μMol ATP) compared to healthy individuals (21.5 ± 2.15 μMol ATP), whereas, exposure of the NIDDM Ca2+ - pumping ATPase to Tp (3mg/ml) or Cp (4mg/ml), increased the affinity of the enzyme for ATP to 22.4±2.32μMol ATP and 22.1±2.22μMol ATP, respectively. Conclusion: These findings suggest that Cp and Tp could therapeutically not only lower blood glucose levels as hypoglycaemic agents but could also attenuate the elevated Ca2+ concentration implicated in diabetes mellitus.en_US
dc.identifier.citationAdisa, R.A., Oke, J.M. and Olorunsogo, O.O. (2017). Modulation of Erythrocyte Ghost Membrane Ca2+-Pumping ATPase of Healthy and Non-Insulin Dependent Diabetic Humans by alpha, alpha Trehalose-6- Phosphate and Extracts of Carica papaya Linn. Nig. Qt J. Hosp. Med. Vol. 27(3).en_US
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7648
dc.language.isoenen_US
dc.publisherNigerian Quarterly Journal of Hospital Medicineen_US
dc.relation.ispartofseriesNig. Qt J. Hosp. Med;Vol.27(3)
dc.subjectResearch Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistryen_US
dc.subjectDiabetes mellitusen_US
dc.subjectCalcium-ATPaseen_US
dc.subjectCarica papayaen_US
dc.subjectTrehalose-6-phosphateen_US
dc.titleModulation of Erythrocyte Ghost Membrane Ca2+-Pumping ATPase of Healthy and Non-Insulin Dependent Diabetic Humans by alpha, alpha Trehalose-6- Phosphate and Extracts of Carica papaya Linn.en_US
dc.typeArticleen_US
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