Exploring the genetic diversity of Eimeria acervulina: A polymerase chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) approach

Simple item page
dc.contributor.authorAdeyemi O.
dc.contributor.authorQuill A.
dc.contributor.authorMorikone M.
dc.contributor.authorEvans L.
dc.contributor.authorFormoy C.
dc.contributor.authorIdowu E.T.
dc.contributor.authorAkinsanya B.
dc.contributor.authorJatau I.D.
dc.contributor.authorBlake D.P.
dc.date.accessioned2023-10-18T13:58:21Z
dc.date.available2023-10-18T13:58:21Z
dc.date.issued2023-08-20
dc.descriptionScholarly article
dc.description.abstractEimeria, protozoan parasites that can cause the disease coccidiosis, pose a persistent challenge to poultry production and welfare. Control is commonly achieved using good husbandry supplemented with routine chemoprophylaxis and/or live parasite vaccination, although widespread drug resistance and challenges to vaccine supply or cost can prove limiting. Extensive effort has been applied to develop subunit anticoccidial vaccines as scalable, cost-effective alternatives, but translation to the field will require a robust understanding of parasite diversity. Using a new Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) panel we begin to describe the genetic diversity of Eimeria acervulina populations in Africa and Europe. PCR-RFLP genotyping E. acervulina populations sampled from commercial broiler and layer chickens reared in Nigeria or the United Kingdom (UK) and Republic of Ireland (RoI) revealed comparable levels of haplotype diversity, in direct contrast to previous descriptions from the close relative E. tenella. Here, 25 distinct PCR-RFLP haplotypes were detected from a panel of 42 E. acervulina samples, including 0.7 and 0.5 haplotypes per sample in Nigeria (n = 20) and the UK/RoI (n = 14), respectively. All but six haplotypes were found to be country-specific. The PCRRFLP markers immune mapped protein 1 (IMP1) and heat shock protein 90 (HSP90) were most informative for Nigerian E. acervulina, while microneme protein 3 (MIC3) and HSP90 were most informative in UK/RoI populations. High haplotype diversity within E. acervulina populations may indicate frequent genetic exchange and potential for rapid dissemination of genetic material associated with escape from selective barriers such as anticoccidial drugs and future subunit vaccines.
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BBSRC) under the projects BB/H009337/1 and BB/L00478X/1. Tertiary Education Trust Fund (TETFUND) of Nigeria through the University of Lagos.
dc.identifier.citationAdeyemi O.; Quill A.; Morikone M.; Evans L.; Formoy C.; Idowu E.T.; Akinsanya B.; Jatau I.D.; Blake D.P. (2023). Exploring the genetic diversity of Eimeria acervulina: A polymerase chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) approach. Veterinary Parasitology 322, pp. 2-8.
dc.identifier.otherhttps://doi.org/10.1016/j.vetpar.2023.110010
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/12652
dc.language.isoen
dc.publisherVeterinary Parasitology
dc.relation.ispartofseriesVeterinary Parasitology; 322
dc.titleExploring the genetic diversity of Eimeria acervulina: A polymerase chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) approach
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Exploring the genetic diversity of Eimeria acervulina_ A polymerase chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) approach.pdf
Size:
799.65 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Zoology-Scholarly Publications