Effects of sodium ion and water hyacinth extract in the production of Curvularia pallescens in culture media
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Date
2009-05
Authors
Okunowo, W.O.
Ogunkanmi, L.A.
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
An investigation was conducted on the pH, nutritional and cost benefit of the use of different substrates
for the production of Curvularia pallescens Boedijn. The growth of the organism was examined at pH
range between 5.5 and 8.6 and on different growth media, potato carrot agar (PCA), potato dextrose
agar (PDA), sabouraud agar (SA), tap water agar (TWA), water hyacinth agar (WHA) and Czapek-Dox
agar (ZA). Also, the growth of the organism was examined on different enrichment media “Czapek-Dox”
with its sodium nitrate substituted with equal amount of different nitrogen sources (ammonium
chloride, ammonium nitrate, glutamine and sodium glutamate). WHA produced significantly (P < 0.001)
highest amount of mycelial growth (84.95 ± 0.05 mm) on day 6 than other media types. The spore
concentration was highest, 6.6 E06 ± 3.33 E05 spores/ml with TWA. The colony size was significantly (P <
0.001) highest, 84.9 ± 0.1 mm on day 10 with sodium glutamate compared with other nitrogen sources.
Spore count was only observed on ammonium chloride (1.67E06 ± 3.33E05 spores/ml), the optimal
growth pH was 5.5. The cost of production of the organism per culture plate on WHA was 61.3 and 82%
lesser than on PDA and SA respectively. The formulated water hyacinth agar medium appeared most
economically feasible for the mycelial production of C. pallescens Boedijn.
Description
Keywords
Curvularia pallescence Boedjin, growth measurement, pH measurement.
Citation
Okunowo, W.O. Ogunkanmi, L.A. 2009. Effects of sodium ion and water hyacinth extract in the production of Curvularia pallescens in culture media. African Journal of Biochemistry Research.3(5): 238-244