TRANSCRIPTIONAL FACTOR INFLUENCE ON OTA PRODUCTION AND THE QUELLING ATTRIBUTE OF SIRNA ON THE OTA PRODUCING STRAINS OF ASPERGILLUS SECTION NIGRI
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Date
2017-09
Authors
Thomas, B.T.
Ogunkanmi, L.A.
Iwalokun, B.A.
Agu, G.C.
Journal Title
Journal ISSN
Volume Title
Publisher
AFRICAN JOURNAL OF CLINICAL AND EXPERIMENTAL MICROBIOLOGY
Abstract
This study determined the influence of some transcriptional factors on ochratoxin A production as well as investigates the
quelling attributes of some designed siRNA on the OTA producing Aspergillus section Nigri using standard recommended
techniques. Results obtained following comparison of the pks gene promoter sequences from 15 isolates depicts differences in
length and homology with the pks gene ranging from 218bp in a strain of the Aspergillus niger to 700bp in Aspergillus
carbonarius. The alignment of the pks gene promoter region revealed that six and two of the aligned genes have Aba A binding
site corresponding to CATTCT and CATTCC respectively while Brl A binding site was absent in all the isolates. Pac C binding
site corresponding to CCTGGC and GCCAAG was also found in two and three of the pks gene promoter region respectively.
The three designed siRNA shows significant impact on OTA inhibitions with no significant statistical differences (80.9, 74.4 and
75.3% for pks_Ia, pks_Ib and pks_Ic respectively) (F= 3.830, p>0.05). It can be concluded that Are A and Aba A are potential
enhancers for ochratoxin A biosynthesis and none of the investigated transcriptional factors is enough for the activation of
ochratoxin A production. However, pks gene was seen as a good target gene for inactivation in order to develop efficient means
for ochratoxin A control using RNA silencing technology.
Description
Keywords
Transcriptional factors, Ochratoxin A, siRNA, Quelling , Aspergillus section Nigri
Citation
Thomas, B.T.,Ogunkanmi, L.A., Iwalokun, B.A., Agu, G.C (2017b) Transcriptional Factor Influence On Ota Production And The Quelling Attribute Of Sirna On The Ota Producing Strains Of Aspergillus Section Nigri African Journal Of Clinical And Experimental Microbiology 18(4): 210-217