An improved method of extracting genomic DNA from preserved tissues of Capsicum annum for PCR amplification

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Ogunkanmi, A. L.
Oboh, B.O.
Onifade, B.
Ogunjobi, A. A.
Taiwo, I. A.
Ogundipe, O.T.
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Eurasian Journal of Biosciences
In this study we present a method for the extraction of genomic DNA from the different tissues of the Pepper (Capsicum annuum). A standard protocol of Dellaporta was reviewed and modified for DNA extraction from the preserved tissues of the Capsicum sample which was believed to contain high level of polysaccharides. The modified protocol employed yielded high quality DNA and was found to be suitable for PCR and RAPD analyses. The procedure was also found to be reliable and suitable where some materials are not available and does not require phenol-chloroform extraction. The method also allowed for the preservation of plant tissues for some days from a locality where storage facilities are not accessible. We also discovered that irrespective of the sources of tissues, a good quality DNA was obtained. The quantity of DNA produced from the fleshy mesocarp tissue was more than the quantity obtained from the seeds of the same weight, this is probably due to the hard nature of the seeds and there may have been no complete breakdown of the cell wall to release the cellular contents
Capsicum, genomic, mesocarp, pepper, polysaccharides
Ogunkanmi AL, Oboh B, Onifade B, Ogunjobi AA, Taiwo IA, Ogundipe OT (2008) An improved method of extracting genomic DNA from preserved tissues of Capsicum annuum for PCR amplification. EurAsia J BioSci 2, 14, 115-119.