Cytotoxicity Effect and Utilization of Mytilus edulis shell in the diet of Clarias gariepinus
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Ayoola, S. O.
Faculty of Agriculture and Forestry, University of Ibadan
Compounded feed has been known to enhance high yield, promotion of growth and enables the fish farmers to produce table size fish within the shortest possible time. Five isonitrogenous diets (100% Dicalciumphosphate (DCP) compounded feed,75% DCP Compounded feed and 25% Mytilus edulis shell, 50% DCP Compounded feed and 50% Mytilus edulis shell, 25% DCP compounded feed and 75% Mytilus edulis shell,100% Mytilus edulis shell were formulated. The Mytilus edulis shell was washed, sun dried for 5 days and later grinded and added to the feed and were fed to 150 juveniles of Clarias gariepinus which were distributed into 15 different plastic bowls. Ten specimens were stocked in each of the plastic bowls and they were fed twice daily for an experimental period of 12 weeks. In the cytotoxicity, five fishes were picked randomly from each triplicate and their blood collected carefully with syringe from the anal region and kept in sample bottles. The blood was smeared on slides and were air-dried for 24 hours, fixed in ethanol for 20 minutes and followed by 10% Giemsa staining. Each fish had 2000 erythrocytes examined and to detect micronuclei in erythrocytes, the slides were analysed using a 1000X oil-immersion lens. Result shows that specimens fed with 100% DCP compounded feed (control feed) had the highest Mean Weight Gain (MWG) (231.67±5.4g) and Specific Growth Rate (SGR) (1.2±0.3). The second best result was shown in specimens fed with 100% Mytilus edulis shell with the highest Average Daily Growth (ADG) (2.63±0.27), Protein Efficiency Ratio (PER) (5.54±0.59) and Average Final Weight (AFW) (353.33±7.63). . The lowest value Feed conversion ratio (FCR) of 2.21±.01 was obtained in T4 (100% Mytilus edulis shell) hence, 100% Mytilus edulis shell recorded the best FCR. The poorest result was shown in specimens fed with 25% Mytilus edulis shell recording MWG (215±13.22g), SGR (1.15±0.09), ADG (2.50±15), PER (5.19±0.38) and AFW (341.67±10.0). In most cases, differences in fish weight or specific growth rate (SGR) were not significant. Results for micronucleus reveals that T1 (25% MES) had the highest number of micronucleated cells while the rest (T2, T3, and T4 ) including the control had no significant differences. Results for BN reveal that T1 had the highest number of BN and T0 while the control had the least number of BN.In this study the results showed Mytilus edulis shell can be use as subtitute for DCP in the diet of Clarias gariepinus.
Cytotoxicity , Utilization , Clarias gariepinus , Mytilus edulis , Research Subject Categories::NATURAL SCIENCES
Ayoola, S. O (2012). Cytotoxicity Effect and Utilization of Mytilus edulis shell in the diet of Clarias gariepinus. Ibadan Journal of Agricultural Research, Vol.8( 5-9), 76-82