The Distribution of Human Leukocyte Antigens Genotypes Among Nigerian Sickle Cell Anaemia Patients
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Date
2016
Authors
Olowoselu, F.O.
Udenze, I.C.
Oyedeji, O.A.
Ocheni, S.
Ebuehi, O.A.T.
Ayanshina, O.A.
Akinyanju, O.O.
Journal Title
Journal ISSN
Volume Title
Publisher
Nigerian Quarterly Journal of Hospital Medicine
Abstract
Background: Human leukocyte antigens (HLA) are
involved in the modulation of immune responses, thus
specific haplotypes, alleles and residues at different loci
have been linked with disease susceptibility. There is no
substantial data regarding HLA allele's linkage to sickle cell
anaemia (SCA) patients from Nigeria.
Objective: This study was carried out to determine the HLA
–A*, -B*, -C*, -DRB1* and -DQB1* allele frequency
distribution among SCA patients of Nigerian origin.
Methods: This was an observational descriptive study of
169 registered SCA patients of the Sickle Cell Foundation
Nigeria who met the European Society for Blood and
Marrow Transplantation (EBMT) criteria for haematopoietic
bone marrow transplantation and they were counseled for
HLA tissue typing along with their siblings to know if there
were any potential match among them that could donate
stem cell for HSCT. Ten milliliters of venous blood were
collected in a sterile Na-EDTA anticoagulant tube from
each consenting subject and samples were collected and
sent in batches to Istituto Mediterraneo Di Ematologia
(IME), Rome, Italy for HLA study. Genomic DNA was
isolated from the lymphocytes through
HLA genotyping for HLA –A*, -B*, -C*, -DRB1*
and -DQB1* loci typing was done using sequence specific
oligonucleotide polymerase chain reaction.
Results: Sixteen alleles were detected at HLA A* locus, 24
alleles at HLA B* locus, 12 alleles at HLA C* locus, 13
alleles at HLA DRB1* locus, and 5 alleles at HLA DQB1*
locus. The allele frequencies of these most common alleles
were ≥ 10-1. The less common alleles have allele
frequencies (AF %) of ≥10-2.
Conclusion: HLA (A*23, A*30 and A*02), (B*53 and B*15),
(C*04 and C*07), (DRB1*15, DRB1*13, DRB1*11 and
DRB1*03) and (DQB1*06, DQB1*05, DQB1*02 and
DQB1*03) are the most frequent alleles among SCA
patients in Nigeria.
Description
Keywords
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry , Sickle cell anaemia , bone marrow transplantation , human leukocyte antigen , sequence specific oligonucleotide polymerase chain reaction , allele
Citation
Olowoselu, F.O., Udenze, I.C., Oyedeji, O.A., Ocheni, S., Ebuehi, O.A.T., Ayanshina, O.A., Akinyanju, O.O. The Distribution of Human Leukocyte Antigens Genotypes Among Nigerian Sickle Cell Anaemia Patients. Nig. Qt J. Hosp. Med. Vol. 26(2) Apr. - June, 2016.