Antinociceptive and anti-arthritic effects of aqueous whole plant extract of Trianthema portulacastrum in rodents: Possible mechanisms of action
| dc.contributor.author | FALADE, T | |
| dc.contributor.author | ISHOLA, I.O | |
| dc.contributor.author | AKINLEYE, M.O | |
| dc.contributor.author | OLADIMEJI-SALAMI, J.A | |
| dc.contributor.author | ADEYEMI, O.O | |
| dc.date.accessioned | 2019-09-30T12:25:32Z | |
| dc.date.available | 2019-09-30T12:25:32Z | |
| dc.date.issued | 2019-03-28 | |
| dc.description.abstract | Ethnopharmacological relevance: Trianthema portulacastrum L. (Aizoaceae) is used in traditional African Medicine for the treatment of various illnesses including dropsy, inflammation and rheumatism. Aim of the study: This study was designed to investigate the anti-nociceptive and anti-arthritic properties of the aqueous whole plant extract of Trianthema portulacastrum (AETP), possible mechanisms of action and characterize some of the active constituents. Materials and methods: Antinociceptive activity was evaluated using the acetic acid-induced writhing and hot plate tests in mice. The carrageenan test was used to induce a transient inflammation while arthritis was induced with complete Freund’s adjuvant (CFA) in rats. On completion of CFA-induced arthritis macroscopic observations, the rats were euthanized to isolate the spleen, liver and limbs for estimation of oxidative stress and histological analysis. Results: AETP (10, 50, or 250 mg/kg; p.o.) produced significant (p < 0.05) and dose-dependent inhibition (41.10, 50.40, and 67.10%, respectively) of writhing response elicited by acetic acid. Also, increased pain threshold of supraspinally mediated nociceptive behaviour, with peak maximum possible effect (MPE) obtained at 250 mg/kg (22.98%; 30 min post-treatment). However, the pre-treatment of mice with Nitro-L-arginine (LNNA) or naloxone reversed AETP-induced antinociception. In another experiment, AETP produced time course inhibition of carrageenan-induced paw oedema with peak effect (50.60%) at 250 mg/kg as well as significant reduction in CFA-induced arthritis by 58.56%, on day 27 and arthritic index (26.84%). Similarly, AETP attenuated CFA-induced MDA generation and deficit in antioxidant enzyme activities. Histological analysis of rat joints revealed a reduction in the synovial hyperplasia and mononuclear infiltration induced by CFA in AETP treated groups. Conclusion: Findings from this study showed that T. portulacastrum possesses anti-nociceptive action through nitrergic and opioidergic signalling as well as anti-arthritic effect through enhancement of antioxidant defense system and inhibition of release or actions of inflammatory mediators. | en_US |
| dc.identifier.citation | Antinociceptive and anti-arthritic effects of aqueous whole plant extract of Trianthema portulacastrum in rodents: Possible mechanisms of action T. Faladea, I.O. Isholaa,∗, M.O. Akinleyeb, J.A. Oladimeji-Salamic, O.O. Adeyemi Journal of Ethnopharmacology 238 (2019) 111831 | en_US |
| dc.identifier.uri | https://ir.unilag.edu.ng/handle/123456789/6225 | |
| dc.language.iso | en | en_US |
| dc.publisher | ELSEVIER | en_US |
| dc.subject | Freund’s adjuvant | en_US |
| dc.subject | Hot plate | en_US |
| dc.subject | Arthritis | en_US |
| dc.subject | Antioxidant | en_US |
| dc.subject | Opoid receptor | en_US |
| dc.subject | Nitric oxide | en_US |
| dc.title | Antinociceptive and anti-arthritic effects of aqueous whole plant extract of Trianthema portulacastrum in rodents: Possible mechanisms of action | en_US |
| dc.type | Article | en_US |