Role of amygdala kisspeptin in pubertal timing in female rats

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dc.contributor.authorAdekunbi, DA
dc.contributor.authorLi, XF
dc.contributor.authorLi, S
dc.contributor.authorAdegoke, OA
dc.contributor.authorIranloye, BO
dc.contributor.authorMorakinyo, AO
dc.contributor.authorLightman, SL
dc.contributor.authorTaylor, PD
dc.contributor.authorPoston, L
dc.contributor.authorO'Bryne, KT
dc.date.accessioned2019-08-31T11:15:02Z
dc.date.available2019-08-31T11:15:02Z
dc.date.issued2017
dc.description.abstractTo investigate the mechanism by which maternal obesity disrupts reproductive function in offspring, we examined Kiss1 expression in the hypothalamic arcuate (ARC) and anteroventral periventricular (AVPV) nuclei, and posterodorsal medial amygdala (MePD) of pre-pubertal and young adult offspring. Sprague-Dawley rats were fed either a standard or energydense diet for six weeks prior to mating and throughout pregnancy and lactation. Male and female offspring were weaned onto normal diet on postnatal day (pnd) 21. Brains were collected on pnd 30 or 100 for qRT-PCR to determine Kiss1 mRNA levels. Maternal obesity increased Kiss1 mRNA expression in the MePD of pre-pubertal male and female offspring, whereas Kiss1 expression was not affected in the ARC or AVPV at this age. Maternal obesity reduced Kiss1 expression in all three brain regions of 3 month old female offspring, but only in MePD of males. The role of MePD kisspeptin on puberty, estrous cyclicity and preovulatory LH surges was assessed directly in a separate group of post-weanling and young adult female rats exposed to a normal diet throughout their life course. Bilateral intra-MePD cannulae connected to osmotic mini-pumps for delivery of kisspeptin receptor antagonist (Peptide 234 for 14 days) were chronically implanted on pnd 21 or 100. Antagonism of MePD kisspeptin delayed puberty onset, disrupted estrous cyclicity and reduced the incidence of LH surges. These data show that the MePD plays a key role in pubertal timing and ovulation and that maternal obesity may act via amygdala kisspeptin signaling to influence reproductive function in the offspring.en_US
dc.identifier.citationAdekunbi DA, Li XF, Li S, Adegoke OA, Iranloye BO, Morakinyo AO, Lightman SL, Taylor PD, Poston L, O'Byrne KT. (2017) Role of amygdala kisspeptin in pubertal timing in female rats. PLoS ONE 12(8): e0183596en_US
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/4974
dc.subjectKisspeptinen_US
dc.subjectPubertyen_US
dc.subjectMaternal obesityen_US
dc.subjectAmygdalaen_US
dc.titleRole of amygdala kisspeptin in pubertal timing in female ratsen_US
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