Identification of single nucleotide polymorphisms in the agouti signaling protein (ASIP) gene in some goat breeds in tropical and temperate climates

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dc.contributor.authorAdefenwa, M. A.
dc.contributor.authorPeters, S.O.
dc.contributor.authorAgaviezor, B.O.
dc.contributor.authorWheto, M.
dc.contributor.authorAdekoya, K.O.
dc.contributor.authorOkpeku, M.
dc.contributor.authorOboh, B.O.
dc.contributor.authorWilliams, G.O.
dc.contributor.authorAdebambo, O.A.
dc.contributor.authorSingh, M.
dc.contributor.authorThomas, B.
dc.contributor.authorDonato, M.
dc.contributor.authorIkhide, G. I.
dc.date.accessioned2019-01-17T07:57:08Z
dc.date.available2019-01-17T07:57:08Z
dc.date.issued2013-05
dc.description.abstractThe agouti-signaling protein (ASIP) plays a major role in mammalian pigmentation as an antagonist to melanocortin-1 receptor gene to stimulate pheomelanin synthesis, a major pigment conferring mammalian coat color. We sequenced a 352 bp fragment of ASIP gene spanning part of exon 2 and part of intron 2 in 215 animals representing six goat breeds from Nigeria and the United States: West African Dwarf, predominantly black; Red Sokoto, mostly red; and Sahel, mostly white from Nigeria; black and white Alpine, brown and white Spanish and white Saanen from the US. Twenty haplotypes from nine mutations representing three intronic, one silent and five missense (p.S19R, p.N35K, p.L36V, p.M42L and p.L45W) mutations were identified in Nigerian goats. Approximately 89 % of Nigerian goats carry haplotype 1 (TGCCATCCG) which seems to be the wild type configuration of mutations in this region of the gene. Although we found no association between these polymorphisms in the ASIP gene and coat color in Nigerian goats, in-silico functional analysis predicts putative deleterious functional impact of the p.L45W mutation on the basic amino-terminal domain of ASIP. In the American goats, two intronic mutations, g.293G[Aand g.327C[A, were identified in the Alpine breed, although the g.293G[A mutation is common to American and Nigerian goat populations. All Sannen and Sahel goats in this study belong to haplotypes 1 of both populations which seem to be the wild-type composite ASIP haplotype. Overall, there was no clear association of this portion of the ASIP gene interrogated in this study with coat color variation. Therefore, additional genomic analyses of promoter sequence, the entire coding and non-coding regions of the ASIP gene will be required to obtain a definite conclusion.en_US
dc.description.sponsorshipSpringer _ Molecular Biology Reportsen_US
dc.identifier.citationAdefenwa, M. A., Peters, S. O., Agaviezor, B. O., Wheto, M., Adekoya, K.O., Okpeku, M., Oboh, B., Williams, G.O., Adebambo, O.A., Singh, M., Thomas, B., De-Donato, M. and Ikhide, G. I. (2013). Identification of single nucleotide polymorphisms in the agouti signaling protein (ASIP) gene in some goat breeds in tropical and temperate climates. Molecular Biology Reports. 40 (7) : 4447 – 4457.en_US
dc.identifier.issn0301 - 4851
dc.identifier.otherE ISSN : 1573 - 4978
dc.identifier.otherDOI 10.1007/s11033-013-2535-1
dc.identifier.urihttp://ir.unilag.edu.ng:8080/handle/123456789/3525
dc.language.isoenen_US
dc.publisherSpringer Sciences _ Molecular Biology Reportsen_US
dc.subjectAmerican goatsen_US
dc.subjectAgoutien_US
dc.subjectCoat coloren_US
dc.subjectNigerian goatsen_US
dc.subjectSNPen_US
dc.titleIdentification of single nucleotide polymorphisms in the agouti signaling protein (ASIP) gene in some goat breeds in tropical and temperate climatesen_US
dc.typeArticleen_US
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