Association of alpha-thalassemia and Glucose-6-Phosphate Dehydrogenase deficiency with transcranial Doppler ultrasonography in Nigerian children with sickle cell anemia
| dc.contributor.author | Ojewunmi, OO | |
| dc.contributor.author | Adeyemo, TA | |
| dc.contributor.author | Oyetunji, AI | |
| dc.contributor.author | Benn, Y | |
| dc.contributor.author | EKPO, MG | |
| dc.contributor.author | Iwalokun, BA | |
| dc.date.accessioned | 2022-09-14T09:36:57Z | |
| dc.date.available | 2022-09-14T09:36:57Z | |
| dc.date.issued | 2021 | |
| dc.description | Scholarly article | en_US |
| dc.description.abstract | Background: Stroke is a devastating complication of sickle cell anemia (SCA) and can be predicted through abnormally high cerebral blood flow velocity using transcranial Doppler Ultrasonography (TCD). The evidence on the role of alpha-thalassemia and glucose-6-phosphate dehydrogenase (G6PD) deficiency in the development of stroke in children with SCA is conflicting. Thus, this study investigated the association of alpha-thalassemia and G6PD(A−) variant with abnormal TCD velocities among Nigerian children with SCA. Methods: One hundred and forty-one children with SCA were recruited: 72 children presented with normal TCD (defined as the time-averaged mean of the maximum velocity: < 170 cm/s) and 69 children with abnormal TCD (TAMMV ≥ 200 cm/s). Alpha-thalassemia (the α-3.7 globin gene deletion) was determined by multiplex gap-PCR, while G6PD polymorphisms (202G > A and 376A > G) were genotyped using restriction fragment length polymorphism—polymerase chain reaction. Results: The frequency of α-thalassemia trait in the children with normal TCD was higher than those with abnormal TCD: 38/72 (52.8%) [α-/α α: 41.7%, α -/α -:11.1%] versus 21/69 (30.4%) [α-/α α: 27.5%, α -/α -:2.9%], and the odds of abnormal TCD were reduced in the presence of the α-thalassemia trait [Odds Ratio: 0.39, 95% confidence interval: 0.20–0.78, p = 0.007]. However, the frequencies of G6PDA− variant in children with abnormal and normal TCD were similar (11.6% vs. 15.3%, p = 0.522). Conclusion: Our study reveals the protective role of α-thalassemia against the risk of abnormal TCD in Nigerian children with SCA. | en_US |
| dc.identifier.citation | Ojewunmi, O. O., Adeyemo, T. A., Oyetunji, A. I., Benn, Y., Ekpo, M. G., & Iwalokun, B. A. (2021). Association of alpha-thalassemia and Glucose-6-Phosphate Dehydrogenase deficiency with transcranial Doppler ultrasonography in Nigerian children with sickle cell anemia. Journal of clinical laboratory analysis, 35(6), e23802. https://doi.org/10.1002/jcla.23802 | en_US |
| dc.identifier.other | doi: 10.1002/jcla.23802 | |
| dc.identifier.uri | https://ir.unilag.edu.ng/handle/123456789/11484 | |
| dc.language.iso | en | en_US |
| dc.publisher | Wiley Periodicals, LLC | en_US |
| dc.subject | Sickle cell anemia | en_US |
| dc.subject | Alpha thalassemia | en_US |
| dc.subject | G-6-PD | en_US |
| dc.subject | TCD | en_US |
| dc.subject | Research Subject Categories::MEDICINE | en_US |
| dc.title | Association of alpha-thalassemia and Glucose-6-Phosphate Dehydrogenase deficiency with transcranial Doppler ultrasonography in Nigerian children with sickle cell anemia | en_US |
| dc.type | Article | en_US |