Isolation and PCR Detection of Cronobacter sakazakii from Powdered Infant Formulae retailed in Nigeria
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Ezeh, R. A.
Aboaba, O. O.
Smith, S. I.
Fesobi, W. A.
Omonigbehin, E. A.
American Journal of Food and Nutrition
The presence of Cronobacter sakazakii in 154 samples of powdered infant formulae (PIF) retailed in Nigeria was analyzed using real time polymerase chain reaction (PCR) and a chromogenic medium, Druggan-Forsythe-Iversen (DFI) agar. Two pairs of species specific primers (SG-F/SG-R and SI-F/SI-R) targeting the sequences between 16S rDNA and 23S rDNA (internal transcribed spacer, ITS) were used for the amplification. Out of the 154 samples analyzed, C. sakazakii was detected in 3 (1.95%). A particular isolate detected by SI primer was not detected by SG primer. This study shows that Cronobacter sakazakii can be detected using both the chromogenic DFI agar (cultural method) and molecular method. This report is the first for the isolation and molecular detection of Cronobacter sakazakii from powdered infant formulae retailed in Nigeria.
Cronobacter sakazakii , powdered infant formula , Research Subject Categories::NATURAL SCIENCES
Rashidat, E., Olugbo, A., Ifeanyl, S., Adetoun, F., Adedayo, O. and Moses, B., 2013. Isolation and PCR detection of Cronobacter sakazakii from powdered infant formulae retailed in Nigeria. Am. J. Food Nutr, 3, pp.182-187.