Isolation and PCR Detection of Cronobacter sakazakii from Powdered Infant Formulae retailed in Nigeria

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dc.contributor.authorEzeh, R. A.
dc.contributor.authorAboaba, O. O.
dc.contributor.authorSmith, S. I.
dc.contributor.authorFesobi, W. A.
dc.contributor.authorOmonigbehin, E. A.
dc.contributor.authorBamidele, M.
dc.date.accessioned2022-09-08T14:22:19Z
dc.date.available2022-09-08T14:22:19Z
dc.date.issued2013-01-01
dc.descriptionScholarly articleen_US
dc.description.abstractThe presence of Cronobacter sakazakii in 154 samples of powdered infant formulae (PIF) retailed in Nigeria was analyzed using real time polymerase chain reaction (PCR) and a chromogenic medium, Druggan-Forsythe-Iversen (DFI) agar. Two pairs of species specific primers (SG-F/SG-R and SI-F/SI-R) targeting the sequences between 16S rDNA and 23S rDNA (internal transcribed spacer, ITS) were used for the amplification. Out of the 154 samples analyzed, C. sakazakii was detected in 3 (1.95%). A particular isolate detected by SI primer was not detected by SG primer. This study shows that Cronobacter sakazakii can be detected using both the chromogenic DFI agar (cultural method) and molecular method. This report is the first for the isolation and molecular detection of Cronobacter sakazakii from powdered infant formulae retailed in Nigeria.en_US
dc.identifier.citationRashidat, E., Olugbo, A., Ifeanyl, S., Adetoun, F., Adedayo, O. and Moses, B., 2013. Isolation and PCR detection of Cronobacter sakazakii from powdered infant formulae retailed in Nigeria. Am. J. Food Nutr, 3, pp.182-187.en_US
dc.identifier.issn2157-1317
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/11378
dc.language.isoenen_US
dc.publisherAmerican Journal of Food and Nutritionen_US
dc.subjectCronobacter sakazakiien_US
dc.subjectpowdered infant formulaen_US
dc.subjectResearch Subject Categories::NATURAL SCIENCESen_US
dc.titleIsolation and PCR Detection of Cronobacter sakazakii from Powdered Infant Formulae retailed in Nigeriaen_US
dc.typeArticleen_US
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