Enzyme-Linked Immunosorbent Assay (Elisa) of Aflatoxin B1 in Groundnut and Cereal Grains in Lagos, Nigeria

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Ayejuyo, O.O
Olowu, R.A
Agbaje, T.O
Atamenwan, M
Osundiya, M.O
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Research Journal of Chemical Sciences
Enzyme linked Immunosorbent assay (ELISA) technique was used to assess the levels of Aflatoxin B1 in Groundnut and Cereal grains commonly consumed by wide majority of Nigeria populace. Homogenous samples purchased from different markets in Lagos were extracted with 70% (v/v) methanol. The extracted samples and HRD-conjugated aflatoxin B1 were mixed and added to the antibody coated micro well. On removal of non-specific reactant, TMB substrate was added and the micro wells measured optically by micro plate reader at 450 nm. The results showed that Aflatoxin contents of Groundnut ranged from 6.25 ng/g to 7.80 ng/g. The levels of this substance were 4.18 ng/g and 28.50 ng/g in millet samples. The maize samples contained between 2.51 ng/g and 3.94 ng/g Aflatoxin as against the 5.20 ng/g found in sorghum. About fifty one percent Aflatoxin incidence was found in the 99 samples investigated. With a safe limit of 20 ng/g set by food and drug administration agency, only the millet samples would prove toxic to the consumers of these products.
Staff publications
Aflatoxin B1 , food products , enzyme linked Immunosorbent assays (ELISA) , Research Subject Categories::NATURAL SCIENCES::Chemistry
Ayejuyo O.O., Olowu R.A., Agbaje T.O., Atamenwan M. & Osundiya M.O. (2011). Linked Immunosorbent Assay (Elisa) of Aflatoxin B1 in Groundnut and Cereal Grains in Lagos, Nigeria. Research Journal of Chemical Sciences, 1(8), 1-5.