Enzyme-Linked Immunosorbent Assay (Elisa) of Aflatoxin B1 in Groundnut and Cereal Grains in Lagos, Nigeria

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dc.contributor.authorAyejuyo, O.O
dc.contributor.authorOlowu, R.A
dc.contributor.authorAgbaje, T.O
dc.contributor.authorAtamenwan, M
dc.contributor.authorOsundiya, M.O
dc.date.accessioned2020-03-02T08:15:38Z
dc.date.available2020-03-02T08:15:38Z
dc.date.issued2011-11
dc.descriptionStaff publicationsen_US
dc.description.abstractEnzyme linked Immunosorbent assay (ELISA) technique was used to assess the levels of Aflatoxin B1 in Groundnut and Cereal grains commonly consumed by wide majority of Nigeria populace. Homogenous samples purchased from different markets in Lagos were extracted with 70% (v/v) methanol. The extracted samples and HRD-conjugated aflatoxin B1 were mixed and added to the antibody coated micro well. On removal of non-specific reactant, TMB substrate was added and the micro wells measured optically by micro plate reader at 450 nm. The results showed that Aflatoxin contents of Groundnut ranged from 6.25 ng/g to 7.80 ng/g. The levels of this substance were 4.18 ng/g and 28.50 ng/g in millet samples. The maize samples contained between 2.51 ng/g and 3.94 ng/g Aflatoxin as against the 5.20 ng/g found in sorghum. About fifty one percent Aflatoxin incidence was found in the 99 samples investigated. With a safe limit of 20 ng/g set by food and drug administration agency, only the millet samples would prove toxic to the consumers of these products.en_US
dc.identifier.citationAyejuyo O.O., Olowu R.A., Agbaje T.O., Atamenwan M. & Osundiya M.O. (2011). Linked Immunosorbent Assay (Elisa) of Aflatoxin B1 in Groundnut and Cereal Grains in Lagos, Nigeria. Research Journal of Chemical Sciences, 1(8), 1-5.en_US
dc.identifier.issn2231-606X
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7926
dc.language.isoenen_US
dc.publisherResearch Journal of Chemical Sciencesen_US
dc.relation.ispartofseriesInternet Journal of Food Safety;Vol.1(8)
dc.subjectAflatoxin B1en_US
dc.subjectfood productsen_US
dc.subjectenzyme linked Immunosorbent assays (ELISA)en_US
dc.subjectResearch Subject Categories::NATURAL SCIENCES::Chemistryen_US
dc.titleEnzyme-Linked Immunosorbent Assay (Elisa) of Aflatoxin B1 in Groundnut and Cereal Grains in Lagos, Nigeriaen_US
dc.typeArticleen_US
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