Detection of efflux pump activity among clinical isolates of Staphylococcus and Micrococcus species

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Adeluola, A.O.
Oyedeji, K.S.
Mendie, U.E.
Johnson, J.R.
Porter, J.R.
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Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Purpose: To detect efflux pump activity (EPA) and screening a suspected efflux pump inhibitor (EPI) [1(3-(trifluoromethyl)benzyl]-piperazine (TFMBP)], which could help in reducing multi-drug resistance (MDR). Methods: Eighteen isolates, viz, 14 S. aureus, 2 S. lentus, 1 S. xylosus and 1 Micrococcus species from various hospital infections of admitted patients were screened for antibiotics susceptibility to 11 classes of antibiotics including oxacillin and β-lactamase production. Efflux pump activity (EPA) was determined by minimum inhibitory concentration (MIC) technique in the presence and absence of TFMBP, the isolates were also screened for MDR genes. Results: All the isolates were resistant to ampicillin (10 µg) and penicillin (10 µg), but sensitive to bacitracin (10 µg). Majority of the isolates were MDR 12/18 (66.7 %), 10 (55.6 %) were inducible βlactamase producers and 3 (16.7 %) were intrinsic β-lactamase producers. Seven (38.9 %) were resistant to oxacillin and also produced carbapenemase enzyme. Eight (66.7 %) of the 12 MDR isolates gave evidence of EPA with TFMBP. However, no MDR genes were detected. Conclusion: Staphylococcus and Micrococcus species exhibit EPA in antibiotic resistance while a suitable EPI such as TFMBP when combined with specific antibiotics could help combat this menace.
Staff publications
[1-(3-(Trifluoromethyl)benzyl]-piperazine , Efflux pump activity , Oxacilline resistant S. aureus , Multidrug resistant , Carbapenemase , Research Subject Categories::PHARMACY::Pharmaceutics
Adeluola, A. O., Oyedeji, K. S., Mendie, U. E., Johnson, J. R., & Porter, J. R. (2017). Detection of efflux pump activity among clinical isolates of Staphylococcus and Micrococcus species. Tropical Journal of Pharmaceutical Research, 16(11), 2697-2704.