Detection of efflux pump activity among clinical isolates of Staphylococcus and Micrococcus species
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Date
2017-11
Authors
Adeluola, A.O.
Oyedeji, K.S.
Mendie, U.E.
Johnson, J.R.
Porter, J.R.
Journal Title
Journal ISSN
Volume Title
Publisher
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Abstract
Purpose: To detect efflux pump activity (EPA) and screening a suspected efflux pump inhibitor (EPI) [1(3-(trifluoromethyl)benzyl]-piperazine (TFMBP)], which could help in reducing multi-drug resistance (MDR). Methods: Eighteen isolates, viz, 14 S. aureus, 2 S. lentus, 1 S. xylosus and 1 Micrococcus species from various hospital infections of admitted patients were screened for antibiotics susceptibility to 11 classes of antibiotics including oxacillin and β-lactamase production. Efflux pump activity (EPA) was determined by minimum inhibitory concentration (MIC) technique in the presence and absence of TFMBP, the isolates were also screened for MDR genes. Results: All the isolates were resistant to ampicillin (10 µg) and penicillin (10 µg), but sensitive to bacitracin (10 µg). Majority of the isolates were MDR 12/18 (66.7 %), 10 (55.6 %) were inducible βlactamase producers and 3 (16.7 %) were intrinsic β-lactamase producers. Seven (38.9 %) were resistant to oxacillin and also produced carbapenemase enzyme. Eight (66.7 %) of the 12 MDR isolates gave evidence of EPA with TFMBP. However, no MDR genes were detected. Conclusion: Staphylococcus and Micrococcus species exhibit EPA in antibiotic resistance while a suitable EPI such as TFMBP when combined with specific antibiotics could help combat this menace.
Description
Staff publications
Keywords
[1-(3-(Trifluoromethyl)benzyl]-piperazine , Efflux pump activity , Oxacilline resistant S. aureus , Multidrug resistant , Carbapenemase , Research Subject Categories::PHARMACY::Pharmaceutics
Citation
Adeluola, A. O., Oyedeji, K. S., Mendie, U. E., Johnson, J. R., & Porter, J. R. (2017). Detection of efflux pump activity among clinical isolates of Staphylococcus and Micrococcus species. Tropical Journal of Pharmaceutical Research, 16(11), 2697-2704.