Detection of efflux pump activity among clinical isolates of Staphylococcus and Micrococcus species

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dc.contributor.authorAdeluola, A.O.
dc.contributor.authorOyedeji, K.S.
dc.contributor.authorMendie, U.E.
dc.contributor.authorJohnson, J.R.
dc.contributor.authorPorter, J.R.
dc.date.accessioned2020-01-07T08:18:49Z
dc.date.available2020-01-07T08:18:49Z
dc.date.issued2017-11
dc.descriptionStaff publicationsen_US
dc.description.abstractPurpose: To detect efflux pump activity (EPA) and screening a suspected efflux pump inhibitor (EPI) [1(3-(trifluoromethyl)benzyl]-piperazine (TFMBP)], which could help in reducing multi-drug resistance (MDR). Methods: Eighteen isolates, viz, 14 S. aureus, 2 S. lentus, 1 S. xylosus and 1 Micrococcus species from various hospital infections of admitted patients were screened for antibiotics susceptibility to 11 classes of antibiotics including oxacillin and β-lactamase production. Efflux pump activity (EPA) was determined by minimum inhibitory concentration (MIC) technique in the presence and absence of TFMBP, the isolates were also screened for MDR genes. Results: All the isolates were resistant to ampicillin (10 µg) and penicillin (10 µg), but sensitive to bacitracin (10 µg). Majority of the isolates were MDR 12/18 (66.7 %), 10 (55.6 %) were inducible βlactamase producers and 3 (16.7 %) were intrinsic β-lactamase producers. Seven (38.9 %) were resistant to oxacillin and also produced carbapenemase enzyme. Eight (66.7 %) of the 12 MDR isolates gave evidence of EPA with TFMBP. However, no MDR genes were detected. Conclusion: Staphylococcus and Micrococcus species exhibit EPA in antibiotic resistance while a suitable EPI such as TFMBP when combined with specific antibiotics could help combat this menace.en_US
dc.identifier.citationAdeluola, A. O., Oyedeji, K. S., Mendie, U. E., Johnson, J. R., & Porter, J. R. (2017). Detection of efflux pump activity among clinical isolates of Staphylococcus and Micrococcus species. Tropical Journal of Pharmaceutical Research, 16(11), 2697-2704.en_US
dc.identifier.issn1596-9827
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7295
dc.language.isoenen_US
dc.publisherPharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.en_US
dc.relation.ispartofseriesTropical Journal of Pharmaceutical Research;Vol.16(11)
dc.subject[1-(3-(Trifluoromethyl)benzyl]-piperazineen_US
dc.subjectEfflux pump activityen_US
dc.subjectOxacilline resistant S. aureusen_US
dc.subjectMultidrug resistanten_US
dc.subjectCarbapenemaseen_US
dc.subjectResearch Subject Categories::PHARMACY::Pharmaceuticsen_US
dc.titleDetection of efflux pump activity among clinical isolates of Staphylococcus and Micrococcus speciesen_US
dc.typeArticleen_US
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