Application of a New Iterative Method To Analysis of Kinetics of Thermal Inactivation of Enzyme
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Date
2018
Authors
Sobamowo, M.G
Adeleye, O.A
Journal Title
Journal ISSN
Volume Title
Publisher
U.P.B. Scientific Bulletin
Abstract
In this paper, a new iterative method proposed by Temimi and Ansari is applied to analyze the kinetic of thermal inactivation of jack bean urease (EC3.5.1.5). The analyzed kinetics or reaction mechanisms consist of three-reaction steps and included the Arrhenius equation for the temperature dependence of rate constants as well as the temperature change in the initial heating period. The obtained solutions are used to study the model parameters on the kinetics of thermal inactivation of enzyme. The analytical solutions are verified with numerical solutions using Runge – Kutta with shooting method and good agreements are established between the solutions. The information given in this theoretical investigation will assist in the kinetic analysis of the experimental results over handling rate constants and molar concentrations.
Description
Staff Publications
Keywords
Kinetics , Thermal Activation , Jack Bean Urease , Enzyme , Research Subject Categories::TECHNOLOGY
Citation
SOBAMOWO, M., & ADELEYE, O. APPLICATION OF A NEW ITERATIVE METHOD TO ANALYSIS OF KINETICS OF THERMAL INACTIVATION OF ENZYM E. Series B, 80(3), 147-164.