Genetic Diversity of Vernonia as Revealed By Random Amplified Polymorphic DNA (RAPD) Markers
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Egyptian Academic Journal of Biological Sciences (H. Botany)
Vernonia Schreb. is a genus in the family Asteraceae. It has over 1000 species which may be trees, shrubs, woody climbers or herbs. Some of the species are economically important as sources of food and herbal medicine as well as for industrial purposes. This study was carried out to assess the genetic diversity of Vernonia species in Nigeria. Molecular characterization was carried out on a total of 50 Vernonia samples consisting of 34 samples (13 species) from field collections and 16 samples (10 species) from Ife Herbarium collections making a total of 23 species using 4 RAPD primers. Analysis of the molecular data was done using NTsys version 2.02j computer programme. Results from molecular studies showed amplification through polymerase chain reaction (PCR) with 4 RAPD markers namely OPA-02, OPD-14, OPC-10 and OPE-01. Analysis of the molecular data grouped the samples into 4 Groups with only one species ungrouped. Group I had 17 species, II had 3 species, III had 6 species while IV had 2 species. V. amygdalina was represented in all 4 Groups, V. glabra and V. guineense were present in Groups 1 and 3, V. tenoreana and V. purpurea were in Groups 1 and 2 respectively, while V. colorata remained ungrouped. All 50 Vernonia samples used in the molecular study clustered at 64% and analyzed at a similarity coefficient of 72%. This analysis revealed that RADP markers are useful tools in assessing the level of genetic diversity in Vernonia.
Genetic Diversity , Molecular Characterization , PCR , RAPD , Vernonia
Nwakanma, N. M. C., Oboh, B.O., Adekoya, K.O., and Ogunkanmi, L. A. (2018). Genetic Diversity of Vernonia as Revealed By Random Amplified Polymorphic DNA (RAPD) Markers. Egyptian Academic Journal of Biological Sciences H. Botany. 9 (1) : 27 - 38