Evaluation of two MiniSTR loci mutation events in five Father-Mother-Child trios of Yoruba origin
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Date
2017
Authors
Awe, E.T.
Akpan, U.U.
Adekoya, K.O.
Journal Title
Journal ISSN
Volume Title
Publisher
Biotechnology Society of Nigeria
Abstract
The robustness of short tandem repeats for use in forensic and paternity depends on their high
polymorphism and mutation rate. This study tried to determine the event of mutation of two
miniSTR loci in the Yoruba population. Blood samples were collected from five father-motherchild trios of Yoruba origin. Two DNA extraction methods, an homemade method and Zymogen
gDNA kit were tested for yield and purity for use in the STR assay. The DNA were amplified and
resolved on 4% Agarose gel. The first DNA extraction method yielded an average DNA
concentration of 1399 ng/µl and while the Kit yielded 984.1 ng/µl; absorbence quotient at
260/280 of 1.78 and 1.55 respectively. Locus D1GATA113 was detected in the father and mother
of two families; A and C. D5S2500 was detected only in the male parent (father) in family D. DNA
extracted using any of the two methods in this study is appriopriate for use in STR mutation assay
but the PCR condition for mutation miniSTR loci among the yoruba still requires extensive
optimization.
Description
Keywords
DNA extraction methods , miniSTR , mutation , Yoruba
Citation
Awe, E.T., Akpan, U.U. and Adekoya, K.O. (2017). Evaluation of two MiniSTR loci mutation events in five Father-Mother-Child trios of Yoruba Origin. Nigerian Journal of Biotechnology 33 : 120 – 124