Assessment of Genetic Diversity among Selected Tomato (Solanum lycopersicum L.) Lines Based on SSR Markers
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Adeyemo, O. A.
JOURNAL OF SCIENTIFIC RESEARCH AND DEVELOPMENT
Genetic diversity in eight Nigeria, two Benin Republic and five United Kingdom cultivated tomato lines was assessed using simple sequence repeat (SSR). Genotyping was carried out with 15 SSRs. Among the markers used, 11 SSR markers were polymorphic. For the SSR analysis, the total number of polymorphic alleles was 33, with a mean of 3.00 and the average polymorphic information content (PIC) was 0.36. The genetic diversity within the lines was considerably moderate (0.31). The Estimates of Rogers’ distance varied from 0.03 to 0.66. The 15 lines were clustered into two major groups and a singleton based on unweighted pair-group method with arithmetic mean (UPGMA) cluster analysis of the SSR-based genetic distance (GD) estimates. Group I included 10 lines from all the geographical regions evaluated, and the group II consists of 4 lines which are essentially from Nigeria. TM09 (UK line) grouped separately forming a singleton. Understanding the genetic diversity among the tomato lines is a good starting point for establishing and maintaining collections, germplasm banks and proper characterisation of Nigerian tomatoes in order to preserve the genetic variability.
Tomato, Genetic diversity, molecular markers, genetic distance
Oyenike Adeyemo and Babafemi Olukanmi. 2015. Assessment of genetic diversity among selected tomato (Solanum lycopersicum L.) lines based on SSR markers. J. Sci. Res. Dev., 15(2): 77-84.