Isolation, Identification and Characterization of Pectinase Producers from Agro Wastes (Citrus sinensis and Ananas comosus)
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Date
2020
Authors
Adeyefa, O.M
Ebuehi, O.A.T.
Journal Title
Journal ISSN
Volume Title
Publisher
World Journal of Agriculture and Soil Science
Abstract
Fruits detached from the living plants cannot repair its cells or defend its tissues against natural deterioration. Pectinases, the first enzymes
to be secreted by fungal pathogens, weakens the plant cell wall and expose other polymers to degradation by hemicellulases and cellulases, are of
significant importance in current biotechnological era, with their applications in various industries. The objective of this study, is to isolate and
characterize pectinase producers from Bacillus subtilis and Aspergilus niger based on their carbon and nitrogen sources, using different assays. Fresh
and rotten orange and pineapple peel were incubated in nutrient broth and the resulting microorganisms Bacillus subtilis and Aspergilus niger were
subculture in pectin agar. Pectinase was extracted with pectin agar incubated at room temperature for 6 days. Clear zones of pectinase were isolated
for secondary screening by estimating polygalacturonase (PG) and pectin lyase (PL) production under solid state fermentation by using the modified
medium. Pectinase activity was analysed by alternating certain parameter, such as temperature and pH. Concentration of D-galacturonic acid and
polygalacturonase were adjusted to investigate pectinase activity. The effect of inoculum size (106
CFU/ml, 105
CFU/ml, 104
CFU/ml and 103
CFU/
ml) for the production of pectinase. From this study, 104
CFU/ml and 103
CFU/ml were optimum inoculum sizes for the production of pectinase for
both rotten and fresh oranges and pineapples respectively with level of significance (p < 0.05). Optimum pectinase activity was recorded at pH 5 and
temperature 35 °C and 30 °C and pH5 and temperature 30 °C and 35 °C for both fresh and rotten oranges and pineapples respectively. Considering
its great significance, with tremendous potential pectinase offers industries, one can conclude that strategic ways to produce maximum pectinase
with low cost production should be studied.
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Scholarly article
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Citation
Adeyefa, O.M and Ebuehi, O.A.T. (2020). Isolation, Identification and Characterization of Pectinase Producers from Agro Wastes (Citrus sinensis and Ananas comosus). World Journal of Agriculture and Soil Science. 4 (3): 1-6