Development of Simple in-vitro Protocol for Screening Low Soil nitrogen-tolerant Maize lines

Simple item page
dc.contributor.authorAkinyosoye, S. T.
dc.contributor.authorAdetumbi, J. A.
dc.contributor.authorOlowolafe, M. O.
dc.contributor.authorOlakojo, S. A.
dc.contributor.authorAmusa, O. D.
dc.date.accessioned2020-01-06T14:20:28Z
dc.date.available2020-01-06T14:20:28Z
dc.date.issued2018
dc.descriptionStaff publicationsen_US
dc.description.abstractIn order to fast track and compliment conventional maize breeding programme designed for low nitrogen (N) environments, seeds of eight low soil N-tolerant maize lines were sown in the field under no nitrogen and 40 kgN/ha for in vivo evaluation with a view to develop simple protocols for screening maize lines for low nitrogen-efficient through tissue culture technique. Maize plants used for tissue culture (in vitro) were selfed, cobs were harvested fourteen days after pollination before the kernels were disinfected. The immature embryos were excised and cultured on modified Murashige and Skoog basal medium (MS) containing 100% Nitrogen (1650 mg/l), 50% Nitrogen (825 mg/l), 25% Nitrogen (412.5 mg/l), 0% Nitrogen of normal ammonium nitrate in MS salt formulation. Results obtained revealed three lines (LNTP-YC6, TZPB Prol C3 and SW5-OB-BPR-1) had higher grain yield than other maize lines under no fertilizer (0 kgN/ha) and 40 kgN/ha fertilizer applications (P = 0.01) while two lines (TZPB Prol C3 and LAPOSTA SEQUIA C6) performed better than others with or without ammonium nitrate under in-vitro condition (P = 0.01). TZPB Prol C3 was consistent in both in-vitro and in-vivo conditions. Also, MS medium without ammonium nitrate appeared to be more suitable for screening for low N-tolerant maize genotypes.en_US
dc.identifier.citationAkinyosoye, S.T., Adetumbi, J.A., Olowolafe, M.O., Olakojo, S.A. and Amusa, O.D. (2018). Development of Simple in-vitro Protocol for Screening Low Soil nitrogen-tolerant Maize lines. Current Plant Biology, 13, 32-35 (Elsevier, USA). doi: 10.1016/j.cpb.2018.03.001en_US
dc.identifier.otherdoi: 10.1016/j.cpb.2018.03.001
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7284
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesCurrent Plant Biology;Vol.13
dc.subjectMaizeen_US
dc.subjectIn vitroen_US
dc.subjectMS mediumen_US
dc.subjectNitrogen regimesen_US
dc.subjectCallus inductionen_US
dc.subjectGrain yielden_US
dc.subjectResearch Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biologyen_US
dc.titleDevelopment of Simple in-vitro Protocol for Screening Low Soil nitrogen-tolerant Maize linesen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Akinyosoye et al. 2018 - Development of Simple in-vitro Protocol for Screening Low Soil nitrogen-tolerant Maize lines..pdf
Size:
355.91 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Cell Biology & Genetics-Scholarly Publications