INTERPERITONEAL VS ORAL ROUTE OF ALCOHOL ADMINISTRATION,WHICH IS BETTER?

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Date
2013-06
Authors
Dawodu, OG
Ebuehi, OAT
Odesanmi, OS
Buari, AS
Journal Title
Journal ISSN
Volume Title
Publisher
Global Research Journals
Abstract
The major problem researchers of alcohol intoxication and its dependence face is the route of administration as animals though good do not mimic the perfect subjects i.e. human beings. This study however compares between 2 accepted means of alcohol intoxication namely interperitoneal (i.p) and oral (o.r) routes. A total of 24 Rats were divided into 4 groups vis-à-vis (a) oral group administered with 63mg (10%) and 130mg (20%) ethanol kg-1 body weight (chronic) for a period of 14 days and concomitantly, the intraperitoneal group, injected with 130mg (20%) ethanol kg-1 body weight for a period of 7 days (acute) and lastly the control group injected with normal saline as the body weight dictated. After this period of intoxication the following biochemical parameters were determined ALT, AST, Cholesterol and Electrolyte balance. Ethanol intoxicated rats (chronic and acute) recorded a significant increase in serum aspartate (AST) and Alanine (ALT) amino transferase activities, cholesterol, and a decrease in electrolyte (potassium) the elevation in total protein was not significant with the i.p group showing the highest elevations. Hence, aspartate (AST) and alanine (ALT) aminotransferase, cholesterol, electrolyte estimation (potassium) may be used as biomarkers for the early diagnosis of ethanol toxicity in human beings.
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Keywords
Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry , alcohol intoxication , Biochemical parameters , ALT , AST
Citation
DAWODU OG, EBUEHI OAT, ODESANMI OS, BUARI AS. Global Research Journal of Microbiology Vol.3(2) pp.24 –29.