Comparative evaluation of direct stool smear and formol-ether concentration methods in the identification of cryptosporidium species

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dc.contributor.authorAkujobi, C.N.
dc.contributor.authorOgunsola, F.T.
dc.contributor.authorIregbu, K.C.
dc.contributor.authorOdugbemi, T.O.
dc.date.accessioned2020-01-06T11:38:44Z
dc.date.available2020-01-06T11:38:44Z
dc.date.issued2005
dc.descriptionStaff publicationsen_US
dc.description.abstractCryptosporidium is a common cause of diarrhoea in patients with Human Immunodeficiency Virus (HIV)/Acquired Immunodeficiency Syndrome (AIDS). Unfortunately this pathogen is not often checked for in Microbiology laboratories because the formal-ether stool concentration method for identification of Cryptosporidium is cumbersome and may not be routinely undertaken in very busy laboratories and in laboratories with inadequate personnel. This study was therefore carried out to compare the outcome of direct stool examination and formal-ether concentration method with the aim of finding a . non-cumbersome method of examining for Cryptosporidium species routinely in stools when it is "indicated. Fresh stool specimens of 193 HIV positive and 200 HIV negative patients (control) attending clinic at the Lagos University Teaching Hospital (LUTH) were processed within two hours of collection using direct stool smear and formal-ether concentration methods. Permanently stained slides were prepared using Kinyoun acid-fast stains. Cryptosporidium oocysts were found in 35 (18.1 %) of HIV seropositive patients using direct stool smear method and in 36 (18-7%) using formol-ether concentration method. There was no statistical difference between the two methods (p > 0.05, xz " 0.012, df = 1 at 95% confidence limit critical ratio" 3.841). No Cryptosporidium was identified in the control (HIV negative) patients using either method. Cryptoliporidlum oocylits can be routinely checked for In the Microbiology laboratories using either direct stool smear or formal-ether concentration stool method with comparable sensitivity.en_US
dc.identifier.citationAkujobi, C.N, Ogunsola, F.T, Iregbu, K.C. and Odugbemi, T.O. (2005). Comparative Evaluation of Direct Stool Smear and Formol-ether Concentration Methods in the Identification of Cryptosporidium Species. Nigerian Journal of Health and Biomedical Sciences, 4: 5-7.en_US
dc.identifier.urihttps://www.google.com/search?q=%C2%B7+Comparative+Evaluation+of+Direct+Stool+Smear+and+Formal-ether+Concentration+Methods+in+the+Identification+of+Cryptosporidium+Species.&oq=%C2%B7+Comparative+Evaluation+of+Direct+Stool+Smear+and+Formal-ether+Concentration+Methods+in+the+Identification+of+Cryptosporidium+Species.&aqs=chrome..69i57.885j0j9&sourceid=chrome&ie=UTF-8
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/7245
dc.language.isoenen_US
dc.relation.ispartofseriesNigerian Journal of Health and Biomedical Sciences;Vol.4
dc.subjectCryptosporidium identificationen_US
dc.subjectDirect stool smearen_US
dc.subjectConcentration methoden_US
dc.subjectResearch Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases::Microbiologyen_US
dc.subjectHIVen_US
dc.titleComparative evaluation of direct stool smear and formol-ether concentration methods in the identification of cryptosporidium speciesen_US
dc.typeArticleen_US
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