Molecular typing of Salmonella spp isolated from food handlers and animals in Nigeria
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Date
2011-01-01
Authors
Smith, S. I.
Fowora, M. N.
Goodluck, H. A.
Nwaokorie, F. O.
Aboaba, O. O.
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Molecular Epidemiology and Genetics
Abstract
A total of 61 isolates of Salmonella spp (made up of 26 clinical isolates and 20 food handler and 15 animal isolates) were typed by RAPD-PCR for the purpose of screening for epidemiologically related isolates. The RAPD -PCR typing method used comprised six primers namely 787, 797, 784, 1254, RAPD 1 and RAPD 2 but 784 and 1254 did not produce discriminatory patterns and so were dropped. From the 61 strains, RAPD fingerprinting with primers RAPD 1, 2 produced 22 and 24 fingerprint patterns respectively. RAPD fingerprinting with primers 787, 797 produced 17, 11 fingerprinting patterns respectively. Combinations of the two RAPD 1 and 2 primers increased the discrimination of Salmonella strains to 32 patterns rather than the other primers used. Primer 797 was the least discriminatory. This study showed that the RAPD 1 and 2 primers would be useful for epidemiological typing of the Salmonella spp in Nigeria.
Description
Scholarly article
Keywords
Salmonella , food handlers , Animals , RAPD-PCR , Research Subject Categories::NATURAL SCIENCES
Citation
Smith, S.I., Fowora, M.A., Goodluck, H.A., Nwaokorie, F.O., Aboaba, O.O. and Opere, B., 2011. Molecular typing of Salmonella spp isolated from food handlers and animals in Nigeria. International journal of molecular epidemiology and genetics, 2(1), p.73.