Molecular typing of Salmonella spp isolated from food handlers and animals in Nigeria

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dc.contributor.authorSmith, S. I.
dc.contributor.authorFowora, M. N.
dc.contributor.authorGoodluck, H. A.
dc.contributor.authorNwaokorie, F. O.
dc.contributor.authorAboaba, O. O.
dc.date.accessioned2022-09-07T09:37:53Z
dc.date.available2022-09-07T09:37:53Z
dc.date.issued2011-01-01
dc.descriptionScholarly articleen_US
dc.description.abstractA total of 61 isolates of Salmonella spp (made up of 26 clinical isolates and 20 food handler and 15 animal isolates) were typed by RAPD-PCR for the purpose of screening for epidemiologically related isolates. The RAPD -PCR typing method used comprised six primers namely 787, 797, 784, 1254, RAPD 1 and RAPD 2 but 784 and 1254 did not produce discriminatory patterns and so were dropped. From the 61 strains, RAPD fingerprinting with primers RAPD 1, 2 produced 22 and 24 fingerprint patterns respectively. RAPD fingerprinting with primers 787, 797 produced 17, 11 fingerprinting patterns respectively. Combinations of the two RAPD 1 and 2 primers increased the discrimination of Salmonella strains to 32 patterns rather than the other primers used. Primer 797 was the least discriminatory. This study showed that the RAPD 1 and 2 primers would be useful for epidemiological typing of the Salmonella spp in Nigeria.en_US
dc.identifier.citationSmith, S.I., Fowora, M.A., Goodluck, H.A., Nwaokorie, F.O., Aboaba, O.O. and Opere, B., 2011. Molecular typing of Salmonella spp isolated from food handlers and animals in Nigeria. International journal of molecular epidemiology and genetics, 2(1), p.73.en_US
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/11344
dc.language.isoenen_US
dc.publisherInternational Journal of Molecular Epidemiology and Geneticsen_US
dc.subjectSalmonellaen_US
dc.subjectfood handlersen_US
dc.subjectAnimalsen_US
dc.subjectRAPD-PCRen_US
dc.subjectResearch Subject Categories::NATURAL SCIENCESen_US
dc.titleMolecular typing of Salmonella spp isolated from food handlers and animals in Nigeriaen_US
dc.typeArticleen_US
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