Effect of Allium sativum extract in combination -with orlistat on insulin resistance and disrupted metabolic hormones in high fat diet induced obese rats
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Date
2021-09-27
Authors
Ebuehi, O.A.T.
Journal Title
Journal ISSN
Volume Title
Publisher
Esevier B.V. on behalf of African Institute of Mathematical Sciences
Abstract
Obesity has increased in epidemic proportions globally and the current antiobesity drugs
have treatment limitations which further reduce treatment benefits. The aim of this study
was to evaluate the effect of Allium sativum and Orlistat on insulin resistance, fatty acid
content and metabolic hormones; leptin, ghrelin and adiponectin in high-fat diet-induced
obese rat model. The sprague-dawley rats used in this study were fed with standard chow
as control diet, Fat emulsion as high-fat diet (HFD), HFD+ Orlistat, HFD+ A. sativum extract
and HFD+ Orlistat+ A. sativum extract. Free fatty acid, serum glucose, insulin, leptin, ghrelin, adiponectin, and Homeostasis model assessment for insulin resistance (HOMA-IR) index were determined using standard methods. HFD significantly increased (P < .001) body
weight, serum glucose, free fatty acid content, serum insulin, HOMA-IR and serum leptin.
Furthermore, HFD reduced the serum levels of ghrelin and adiponectin when compared to
control rats. The combined administration of A. sativum and orlistat resulted in a decrease
in the body weight and HOMA-IR and also improved serum levels of glucose, insulin, leptin, ghrelin and adiponectin in obese rats fed with HFD. This suggests A. sativum in combination with orlistat, has the potential to ameliorate insulin resistance and metabolic hormones disrupted by obesity
Description
Keywords
Obesity, Leptin, Insulin, Ghrelin A. sativum Orlistat, High-fat diet
Citation
Ogunyemi Bukola Carolinea,∗ , Osaretin A.T. Ebuehi a , Oshanupin Adeola Cecilia b , Ogunyemi Adewale Kayodec a Department of Biochemistry, College of Medicine, University of Lagos, Lagos State, Nigeria b Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Lagos, Lagos State, Nigeria c Department of Microbiology, Faculty of Science, University of Lagos, Lagos State, Nigeria