Isolation and characterization of compounds from Argemone mexicana L. (Papaveraceae) with Peroxisome Proliferator-Activated Receptor (PPAR)
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Date
2025-03-31
Authors
Oyawaluja, A.A.
Oyawaluja, B.O.
Oiseoghaede, J.O.
Odukoya, O.A.
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West African Journal of Pharmacy/West African Postgraduate College of Pharmacy
Abstract
Background: Argemone mexicana is used as emetic, demulcent, and laxative in folkloric medicine. This study aimed at isolating the chemical compounds and exploring the PPAR modulatory effects of isolated phytochemicals.
Objective: The study was carried out to isolate the chemical compounds and to explore the PPAR modulatory effects of the isolated phytochemicals.
Method: The leaves were collected, authenticated, and voucher specimen deposited at the University of Lagos herbarium, Nigeria. The leaves extracted with ethanol and extracts subjected to column chromatography on sephadex LH-20 and Silica gel. Structure lucidation were achieved by analyses of their 1-Dimensional and 2-Dimensional NMR. Reporter gene assay were performed on the extracts and isolated compounds for activation of PPARα and PPARγ.
Result: Nine compounds were isolated from the ethanol extract of the plant, and were proposed to be: 1(1-(β-d-ribofuranosyl)-1H-1,2,4-triazone), 2a((Z)-2-(2,4-dihydroxy-2,6,6-trimethylcyclohexylidene) acetic acid), 2b(cis-3,6-dihydroxy-a-ionone), 3((E)-4-(r-1’,t-2’,c-4’-trihydroxy-2’,6’,6’- trimethylcyclohexyl) but-3-en-2-one), 4(Rhein), 5(Tamarexetin), 6(41-methoxylquercetin 3-β-D-glucoside), (Tamarixetin-3-O-β-D- robinoside) and 8(Palmitic acid). The extracts were found to activate PPARα with a fold induction of more than 2.0 over the vehicle control but a lower induction was observed with PPARα. The isolated compounds showed lower activation of PPARα and PPARγ.
Conclusion: Three flavonoids, palmitic acid, Rhein, a triazole and three substituted cyclohexane were isolated, while the extract showed PPARα activation, the isolated compounds did not. These effect might be due to the synergistic effect of the constituents, thus the extract has more promising effect.
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Oyawaluja, A. A., Oyawaluja, B. O., Oiseoghaede, J. O., & Odukoya, O. A. (2025). Isolation and characterization of compounds from Argemone mexicana L. (Papaveraceae) with Peroxisome Proliferator-Activated Receptor (PPAR).