Impact of maternal DNA contamination of fetal DNA in chorionic villi on prenatal diagnosis of sickle cell anemia

Simple item page
dc.contributor.authorOloyede, O.A.
dc.contributor.authorTaiwo, O.A.
dc.contributor.authorMinari, J
dc.contributor.authorAdekoya, K.O.
dc.contributor.authorLamina, M
dc.date.accessioned2019-01-14T12:10:46Z
dc.date.available2019-01-14T12:10:46Z
dc.date.issued2017-07-03
dc.description.abstractObjective: The study aim was to determine the hemoglobin genotypic and allelic distributions in fetal population, and to quantitatively evaluate the effect of heterozygous maternal DNA contamination of homozygous fetal DNA in chorionic villi, on fetal hemoglobin genotypes. Materials and Methods: A descriptive, cross sectional study of amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) analysis of fetal hemoglobin genotype using DNA from chorionic villi and maternal venous blood. The primary fetal hemoglobin genotypes were obtained in the first phase and the secondary hemoglobin genotypes after contaminating homozygous primary genotypes (HbAHbA and HbSHbS) with varying proportions of heterozygous maternal DNA (HbAHbS). Data analysis was done with Micosoft Excel 2010 statistical package and Chi-square (goodness-of-fit). Results: There was no statistically significant deviation in the hemoglobin genotypic and allelic counts between the observed and the expected counts in the fetal population based on Mendelian expectation. Contaminating homozygous fetal DNA with >11.1% (0.5 µl) of heterozygous maternal DNA produced significant change in fetal hemoglobin genotype results. Homozygous hemoglobin genotypes HbAHbA were affected more than HbSHbS. Conclusion: Study established Mendelian distribution in the fetal population and the levels of heterozygous maternal contamination of homozygous fetal DNA that resulted in significant risk of misdiagnosis.en_US
dc.description.sponsorshipTropical Journal of Obstetrics and Gynaecologyen_US
dc.identifier.citationOloyede, O.A., Taiwo, O.A., Minari, J., Adekoya, K.O. and Lamina, M. (2017). Impact of maternal DNA contamination of fetal DNA in chorionic villi on prenatal diagnosis of sickle cell anemia. Tropical Journal of Obstetrics and Gynaecology. 34 (1) : 11 - 15en_US
dc.identifier.otherDOI: 10.4103/TJOG.TJOG_22_17
dc.identifier.urihttp://ir.unilag.edu.ng:8080/handle/123456789/3506
dc.language.isoenen_US
dc.publisherWolters Kluwer - Medknowen_US
dc.subjectARMS-PCRen_US
dc.subjectfetalen_US
dc.subjectmaternal contaminationen_US
dc.subjectsickle cell anemiaen_US
dc.titleImpact of maternal DNA contamination of fetal DNA in chorionic villi on prenatal diagnosis of sickle cell anemiaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Impact of maternal DNA contamination of fetal DNA in chorionic villi on prenatal diagnosis of sickle cell anemia.pdf
Size:
595.77 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Cell Biology & Genetics-Scholarly Publications