Rapid Isolation of Genome DNA Suitable for PCR from Tropical Almond (T. catappa) Plant Populations

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dc.contributor.authorOboh, B.O.
dc.contributor.authorOgunkanmi, L. A.
dc.contributor.authorAgwu, N.
dc.date.accessioned2019-09-05T12:48:10Z
dc.date.available2019-09-05T12:48:10Z
dc.date.issued2009
dc.description.abstractThis study was conducted to develop a rapid and efficient method for the isolation of genomic DNA from the tropical woody tree, Terminalia catappa L. Fresh young leaves from 35 trees were sampled for the extraction of genomic DNA. The methodology employed excluded the use of liquid nitrogen and an ultracentrifuge with various modifications in the quantities and reagents used. The result of the extraction showed that genomic DNA of good quality and quantity with a spectrophotometric ratio of between 1.7-2.0 for the trees sampled. Result further showed that the extracted DNA on 1% agarose gel had high molecular weight bands following electrophoresis. Thus, we concluded that the modified protocol used for the extraction of genomic DNA in T. catappa which can be easily adapted to other crops produced DNA of good quality and quantity which can be used for PCR based studies.en_US
dc.identifier.citationOboh, B. O., Ogunkanmi, L. A., & Agwu, N. (2009). Rapid isolation of genome DNA suitable for PCR from tropical almond (T. catappa) plant populations. Int. J. Bot, 5(3), 250-254.en_US
dc.identifier.issn1811-9700
dc.identifier.otherDOI: 10.3923/ijb.2009.250.254
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/5230
dc.language.isoenen_US
dc.publisherAsian Network for Scientific Informationen_US
dc.subjectTerminalia catappa, genomic DNA, absorbance, electrophoresis, agarose, ultracentrifugeen_US
dc.titleRapid Isolation of Genome DNA Suitable for PCR from Tropical Almond (T. catappa) Plant Populationsen_US
dc.typeArticleen_US
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