Methylene tetrahydrofolate reductase and methionine synthase gene polymorphisms as genetic determinants of pre-eclampsia.

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dc.contributor.authorOsunkalu, VO.
dc.contributor.authorTaiwo, CC.
dc.contributor.authorQuao, RA.
dc.date.accessioned2020-11-24T09:59:11Z
dc.date.available2020-11-24T09:59:11Z
dc.date.issued2020-02-06
dc.descriptionScholarly articlesen_US
dc.description.abstractBACKGROUND:Pre-eclampsia(PE)isaleadingcauseofmaternalandneonatalmortalityinAfrica;andhasbeen associatedwiththeinterplayofgenetic,metabolicandenvironmentalfactors.Polymorphismsofmethylenetetrahydrofolatereductase(MTHFR)andmethioninesynthase(MTR)folatecyclegenes,havebeencon-troversiallyassociatedwithpre-eclampsiainstudiesfromdifferenthumanpopulations.Objectives: To determine the distribution of MTHFR C677T and MTR A2756G polymorphisms in a Nigerian population and evaluate possible associations with the occurrence of pre-eclampsia and homocysteine metabolic derangement.MATERIALSANDMethods:ThisstudywasahospitalbasedstudycarriedoutinLagos,South-westernNigeria.Two hundredpregnantwomenclinicallydiagnosedwithpre-eclampsia(studygroup)and200apparentlyhealthy non-pre-eclampticpregnantwomen(controlgroup)wererecruitedforthestudyafterwritteninformedconsent.Pre-eclampsiawasdiagnosedbasedontheInternationalSocietyfortheStudyofHypertensioninPregnancyre-classificationof2013.MTHFRC677TandMTRA2756Gpolymorphismsweredeterminedbypolymerasechain reaction-restrictionfragmentlengthpolymorphism(PCR-RFLP)method.Statistical analyzes were performed using SPSS version 23. Hardy-Weinberg distribution were tested with χ2test.Logisticregressionmodelwasusedtoevaluatetherelationshipofvariableswithpre-eclampsia.Avalueof p < 0.05 was considered statisticallysignificant.Results: MTHFR genotype frequencies of CC, CT and TT were 59.8%; 31.2% and 9.0% in study group and 76.6%;22.3% and 1.0% in the control group respectively. MTR A2756G genotype frequencies of AA, AG and GGgenotypes were 71.9%; 20.1% and 8.0% for the study group and 81.5%; 16.4% and 2.1% for the control group.Occurrence of pre-eclampsia was significantly associated with presence of T allele of MTHFR (OR = 1.855;p < 0.05) and G allele of MTR genes (OR = 1.269; p < 0.05), Homozygosity of TG haplotype significantly increased the occurrence of pre-eclampsia among Nigerian women (OR = 2.252; p < 0.05). Population at-tributable risk fraction percent for the T and G alleles were 16.4% and 11.5% respectively. Mean plasma Hcy level was not, however, significantly affected by MTHFR/MTR haplotypes (F = 1.54; p = 0.157).Conclusion: MTHFR C677T and MTR A2756G polymorphisms were associated with pre-eclampsia in a popula-tionofpregnantwomeninLagos,Nigeriaen_US
dc.identifier.citationOsunkalu VO, Taiwo IA, Makwe CC and Quao RA (2020). Methylene tetrahydrofolate reductase and methionine synthase gene polymorphisms as genetic determinants of pre-eclampsia. Pregnancy Hypertension. 20 (2020) 7-13. https://doi.org/10.1016/j.preghy.2020.02.001en_US
dc.identifier.otherhttps://doi.org/10.1016/j.preghy.2020.02.001
dc.identifier.urihttps://ir.unilag.edu.ng/handle/123456789/8990
dc.language.isoenen_US
dc.subjectPre-eclampsiaen_US
dc.subjectmethylene tetrahydrofolate reductase geneen_US
dc.subjectPolymorphismen_US
dc.subjectMethionine synthase gene polymorphismen_US
dc.subjectFolate metabolismen_US
dc.subjectResearch Subject Categories::MEDICINEen_US
dc.titleMethylene tetrahydrofolate reductase and methionine synthase gene polymorphisms as genetic determinants of pre-eclampsia.en_US
dc.typeArticleen_US
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