Anti-oxidant and In-Vitro Anti-Diabetic and Antioxidant Activities of Ethanol Extract of The Leaf of Nephrolepis Exaltata (L.) Schott. (Nephrolepidaceae)
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Date
2023-12-01
Authors
Oyawaluja, A.A.
Oiseoghaede, J.O.
Odukoya, O.A.
Bello, N.B.
Samuel T.A.
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Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu state, Nigeria
Abstract
Diabetes is one of the non-communicable diseases growing rapidly worldwide with increasing prevalence in children adolescence and young adults. The study of alpha–amylase and alpha glucosidase inhibitory activities of agents provides an insight of their possible antidiabetic properties. The enzymes α–amylase and α-glucosidase are responsible for postprandial glucose levels. The aim of this study was to investigate the antioxidant and inhibitory potentials of the ethanol leaf extract of Nephrolepis exaltata (L.) Schott. (Nephrolepidaceae) against α–amylase and α glucosidase in vitro. DPPH (1,1 diphenyl-2-picryl hydrazyl) free radical scavenging activity and lipid peroxidation assay were used to assess the antioxidant assay of N. exaltata using ascorbic acid
as standard. Alpha-amylase and alpha-glucosidase inhibitory activities were evaluated. Preliminary phytochemical screening showed the presence of phenols, tannins, flavonoids, alkaloids, resins, saponins and carbohydrate. The inhibitory concentration (IC50) for DPPH assay was 5.83 μg/ml and 0.04 μg/ml for anti-lipid peroxidation assay were obtained for the radical scavenging activities, while IC50 of 0.05 μg/ml and 0.03 μg/ml were obtained for the α-amylase and α-glucosidase inhibitory assay respectively. The ethanol leaf extract of Nephrolepis exaltata possesses antioxidant activities useful and inhibited α-amylase and α–glucosidase which are responsible for postprandial glucose levels.
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Oyawaluja, A.A., Oiseoghaede, J.O., Odukoya, O.A., Bello, N.B. and Samuel T.A. (2023). Anti-oxidant and In-Vitro Anti-Diabetic and Antioxidant Activities of Ethanol Extract of The Leaf of Nephrolepis Exaltata (L.) Schott. (Nephrolepidaceae). Trends in Natural Products Research 4(2): 45-51