Department of Medical Microbiology and parasitology

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Browsing Department of Medical Microbiology and parasitology by Author "Adeogo, B.O."

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    Open Access
    Schematic method for effective identification of anaerobes from infected surgical wounds
    (2009) Niemogha, M.T.; Atoyebi, O.A.; Ogunsola, F.T.; Adeogo, B.O.; Egbuna, K.N.; Gbajabiamila, T.A.; Odugbemi, T.
    Objectiye: To investigate post surgical wound Infections for anaer.obes and to develop effective method for their rapid identification. · Method: Fifty seven (57) samples were collected In cooked meat broth, they consisted of 40 wound swabs, 7 pus, 6 vaginal and 4 rectal swabs. They were Inoculated Into two compounded media; Neomycin Blood Agar (NBA) and Neomycin Plasma Agar (NPA) Incubated anaerobically at 37°C for (24-48) hours. Isolated anaerobes were gram-stained and tested using discs Impregnated with antibiotics, bile salts and dyes, carbohydrate fermentation and other standard tests were carried out. Results: Abdominal surgery had the highest anaerobes (44%), Musculoskeletal (25%), Splenectomy and related procedures "(5%). Severe outcomes associated with anaerobes were amputation of limbs and death. Anaerobes were classified Into frag/1/s group, blle resistant, bile sensitive and Fusobacter/um group. Further tests revealed Bacteroides fragllls, Fusobacter/um nucleatfum, Porphyromonas assacharofytica, Prevotef/a intermedia, and Peptosterptococcus magnus as most frequently Isolated, The developed scheme Interlinked the various Identification steps: 1'1 level; Inoculation and growth, 2"" level; Gram .differentiation and 3"' level; Biochemical characterization. Conclusion: The study has shown anaerobic cultures as essential In the management of patients with post surgical would Infections. The scheme serves as a guide of Idealized representation to identification of anaerobes.