Liquid Chromatographic Technique for the Simultaneous Determination of Sulphamethoxazole and Trimethoprim in Pharmaceutical Formulations
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Date
2016
Authors
Olusegun, A.O
Hamed, N.C
Olajire, M
Oladipo, O.J
Journal Title
Journal ISSN
Volume Title
Publisher
University of Lagos Press, Akoka
Abstract
This paper describes the development and validation of a simple, specific, precise, and
accurate Liquid Chromatographic method for the simultaneous determination of
sulphamethoxazole and trimethoprim in pharmaceutical dosage forms. The
chromatographic resolution was achieved with 50mM sodium phosphate buffer and
acetonitrile (85:15) on a reversed phase column - Octyldecylsilane C18 column (100 x
4.6 mm, i.d., 5μm) - at ambient temperature. The flow rate through the column was
1ml/min and the UV detection was at 260nm using Agilent HPLC 1100LC System. The
mean retention times for trimethoprim and sulphamethoxazole were 2.998 and 6.205
minutes respectively. Calibration curves were rectilinear over the ranges 5–80 mg/L
(trimethoprim) and 25–400 mg/L (sulphamethoxazole). The RSD was less than 2.61%
and percentage recovery was between91.93% - 103.98% with respect to precision and
accuracy. The method has been used to analyze brands of cotrimoxazole tablets. The
percentage content of sulphamethoxazole and trimethoprim were found to be comparable
with BP 2002 requirement. This method, which has a fair run time of 6 minutes, is cost
effective for routine analytical work and for quality control and product monitoring.
Keywords:
Description
Journal Articles
Keywords
Sulphamethoxazole , Trimethoprim , HPLC , Pharmaceutical formulations
Citation
Olusegun, A.O, Hamed, N.C, Olajire, M and Oladipo, O.J (2016). Liquid Chromatographic Technique for the Simultaneous Determination of Sulphamethoxazole and Trimethoprim in Pharmaceutical Formulations. Unilag Journal of Medicine, Science and Technology, Vol.4(2), 120-132p.