Kinetics Analysis of Thermal Inactivation of Enzyme used in Biotechnology using Variation Iteration Method
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Date
2018
Authors
Sobamowo, M.G
Adeleye, O.A
Journal Title
Journal ISSN
Volume Title
Publisher
Annals of the Faculty of Engineering Hunedoara
Abstract
In this work, variation iteration method is used to develop analytical solutions for the prediction of molar
concentration of native and denatured jack bean urease (EC 3.5.1.5) through the three–reaction steps kinetic model
of thermal inactivation of the urease. The obtained analytical solutions are used to study the kinetics of thermal
inactivation of the enzyme as applied in biotechnology. The analytical solutions are verified with numerical solutions
using Runge–Kutta with shooting method and good agreements are established between the solutions. The
information given in this theoretical investigation will assist in the kinetic analysis of the experimental results over
handling rate constants and molar concentrations. The analytical solutions as developed in this work can serve as a
starting point for a better understanding of the relationship between the physical quantities of the problems.
Description
Staff Publications
Keywords
Kinetics , Thermal Activation , Enzyme , Jack Bean Urease , Research Subject Categories::TECHNOLOGY
Citation
Sobamowo, G., and Adeleye, O. (2018). Kinetics Analysis of Thermal Inactivation of Enzyme used in Biotechnology using Variation Iteration Method. Annals of the Faculty of Engineering Hunedoara, Vol.16(3), 83-88pp.