The characterization of Nigerian varieties of pepper, Capsicum annuum and Capsicum frutescens by SDS-polyacrylamide gel electrophoresis of seed proteins
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Date
1999
Authors
Odeigah, P. G. C.
Oboh, B.O.
Aghalokpe, I. O.
Journal Title
Journal ISSN
Volume Title
Publisher
Kluwer Academic PublisherS
Abstract
The possibility of using electrophoresis to characterize varieties of pepper, Capsicum annuum and Capsicum frutescens cultivated in Nigeria was investigated. The SDS-polyacrylamide gel electropherogram of extracted total seed proteins of 10 breeding lines in each of the 6 varieties investigated, revealed a pattern in which 12 polypeptide bands with apparent molecular weight range of 22 to 98 kilodaltons could be distinguished. The result showed that the six varieties could be characterized on the basis of presence/absence and staining intensities of 7 polypeptide bands. It is suggested that SDS-polyacrylamide gel electrophoresis of seed proteins provides a useful analytical technique for the characterization of varieties of pepper and there may be genotype duplicates in the collection of Nigerian Capsicum germplasm.
Description
Keywords
Capsicum spp., varieties, pepper, polyacrylamide gel electrophoresis, polypeptides, seed proteins
Citation
Odeigah, P. G. C., Oboh, B., & Aghalokpe, I. O. (1999). The characterization of Nigerian varieties of pepper, Capsicum annuum and Capsicum frutescens by SDS-polyacrylamide gel electrophoresis of seed proteins. Genetic Resources and Crop Evolution, 46(2), 127-131.